Protein Info for AMB_RS10775 in Magnetospirillum magneticum AMB-1

Annotation: CoA transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 60% identical to SMTA_CHLAU: Succinyl-CoA--L-malate CoA-transferase alpha subunit (smtA) from Chloroflexus aurantiacus

KEGG orthology group: None (inferred from 100% identity to mag:amb2133)

MetaCyc: 60% identical to succinyl CoA:L-malate CoA transferase alpha subunit (Chloroflexus aurantiacus)
2.8.3.7-RXN [EC: 2.8.3.22]; 2.8.3.22 [EC: 2.8.3.22]

Predicted SEED Role

"L-carnitine dehydratase/bile acid-inducible protein F (EC 2.8.3.16)" (EC 2.8.3.16)

MetaCyc Pathways

superpathway of the 3-hydroxypropanoate cycle (13/18 steps found)
3-hydroxypropanoate cycle (9/13 steps found)
oxalate degradation II (1/3 steps found)
itaconate degradation (1/4 steps found)

Isozymes

Compare fitness of predicted isozymes for: 2.8.3.16

Use Curated BLAST to search for 2.8.3.16 or 2.8.3.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W5D8 at UniProt or InterPro

Protein Sequence (402 amino acids)

>AMB_RS10775 CoA transferase (Magnetospirillum magneticum AMB-1)
MAHPKRPLDGIRVLDIATFIAAPYAAAILGEFGAEVIKVEQPGGGDTFRRFGSITERDGD
SLMWLSEARNKNSVTLDLRKPEGRELFLKLVARTDVIAENFRPGTLEKWGLGWEELRRVN
PGLILLRISGYGQTGPYKDRPGFARIAHAFGGLSYLAGMPGDVPVTPGSTSLADYMSGLY
GAIGVLLALRHRDATGEGQVIDLALYESVFRALDEIAPAFAMFGKVREREGAGTVNACPH
GHFRCGDGKWVALACTTDRMFARLAEAMERPELADGAKWGLLRNRLAERADVDALVGHWA
ATRPRETVMELCLDFEVPAAPLNTIADIFEDPQFQARGNLLEVADAVLGGVVVPGVVPKL
SATPGRVDHLGPRLGEDNARVYGGLLGLGEAEIAELKSKGVI