Protein Info for AMB_RS10200 in Magnetospirillum magneticum AMB-1

Annotation: 4-hydroxybenzoyl-CoA reductase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03194: 4-hydroxybenzoyl-CoA reductase, alpha subunit
  • amino acids 15 to 762 (748 residues), 1350.1 bits, E=0
• PF01315: Ald_Xan_dh_C
  • amino acids 25 to 131 (107 residues), 112.8 bits, E=1.6e-36
• PF02738: MoCoBD_1
  • amino acids 147 to 392 (246 residues), 237.1 bits, E=2.4e-74
• PF20256: MoCoBD_2
  • amino acids 418 to 703 (286 residues), 244.1 bits, E=3.2e-76

Best Hits

Swiss-Prot: 92% identical to HCRA_THAAR: 4-hydroxybenzoyl-CoA reductase subunit alpha (hcrA) from Thauera aromatica

KEGG orthology group: K04108, 4-hydroxybenzoyl-CoA reductase subunit alpha [EC: 1.3.7.9] (inferred from 100% identity to mag:amb2020)

MetaCyc: 92% identical to 4-hydroxybenzoyl-CoA reductase alpha subunit (Thauera aromatica)
OHBENZCOARED-RXN [EC: 1.1.7.1]

Predicted SEED Role

"Xanthine dehydrogenase, molybdenum binding subunit (EC 1.17.1.4)" in subsystem Purine Utilization (EC 1.17.1.4)

MetaCyc Pathways

• inosine 5'-phosphate degradation (3/4 steps found)
• adenosine nucleotides degradation II (3/5 steps found)
• adenosine nucleotides degradation I (5/8 steps found)
• guanosine nucleotides degradation I (2/4 steps found)
• guanosine nucleotides degradation II (2/4 steps found)
• guanosine nucleotides degradation III (2/4 steps found)
• phenol degradation II (anaerobic) (2/4 steps found)
• purine nucleotides degradation II (aerobic) (6/11 steps found)
• ureide biosynthesis (3/7 steps found)
• purine nucleobases degradation II (anaerobic) (15/24 steps found)
• 4-coumarate degradation (anaerobic) (2/6 steps found)
• 4-ethylphenol degradation (anaerobic) (2/6 steps found)
• superpathway of guanosine nucleotides degradation (plants) (2/6 steps found)
• purine nucleotides degradation I (plants) (6/12 steps found)
• caffeine degradation III (bacteria, via demethylation) (1/7 steps found)
• theophylline degradation (1/9 steps found)
• caffeine degradation IV (bacteria, via demethylation and oxidation) (1/10 steps found)
• superpathway of purines degradation in plants (6/18 steps found)
• anaerobic aromatic compound degradation (Thauera aromatica) (11/27 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.1.4, 1.3.7.9

Use Curated BLAST to search for 1.1.7.1 or 1.17.1.4 or 1.3.7.9

Sequence Analysis Tools

See Q2W5Q1 at UniProt or InterPro

Protein Sequence (769 amino acids)

>AMB_RS10200 4-hydroxybenzoyl-CoA reductase subunit alpha (Magnetospirillum magneticum AMB-1)
MSPKFPKNGTVGARTPLVDGVEKVTGKAKYTADIAAPGALVGRILRSPVAHARIISIDTS
AAEALDGVYAVCTGAETPVPYGVLPIAENEYPLARDKVRYRGDPVAAVAAVDELTAEQAL
NLIKVEYEVLPAYMTPKAAMKEGAIALHDDKPNNVLREVHAEFGDVEAAFGEAELIREKV
YTFAEVNHVHMELNATLAEYDPVRDMLTLNTTTQVPYYVHLKVAACLQMDSARIRVIKPF
LGGGFGARTECLHFEIIAGLLARKAKGTVKLVQTREETFIAHRGRPWTEVKMKIGLMRDG
KIAALALEATQAGGAYAGYGIITILYTGALMHGLYHIPAIKHDAWRVYTNTPPCGAMRGH
GTVDTRAAFEALLTEMGEELGIDSLDIRQRNMLPQIPYTTMYAQKVMSYGVPECLEKVKA
ASGWAERKGKMPKGRGLGIALSHFVSGTSTPKHWTGEPHATVNLKLDFDGGITLLTGAAD
IGQGSNTMATQVAAEVLGVRMSRIRVISADSALTPKDNGSYSSRVTFMVGNASISAAEEL
KAILVKAAAKKLDAREEDIEVIDEVFMVAGSQDPGLTFQEVVKAALIDTGTITVKGTFTC
PTEFQGDKKIRGSAIGATMGFCYAAQVVEASVDEITGRVTAHKVWVAVDVGKALNPLAVE
GQTQGGVWMGMGQALSEETRWDDGKMMHGNILDYRVPTMAESPDIEVMIVESLDPNGPFG
AKEASEGMLAGFLPAVHEAVYEAVGVRSTKFPLSPETITELLDAKEAAE