Protein Info for AMB_RS09835 in Magnetospirillum magneticum AMB-1

Annotation: KR domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00109: ketoacyl-synt
  • amino acids 9 to 259 (251 residues), 202.5 bits, E=5.4e-63
• PF02801: Ketoacyl-synt_C
  • amino acids 267 to 384 (118 residues), 127.2 bits, 2e-40
• PF16197: KAsynt_C_assoc
  • amino acids 387 to 499 (113 residues), 47.1 bits, 1.8e-15
• PF22621: CurL-like_PKS_C
  • amino acids 451 to 514 (64 residues), 57.5 bits, see alignment (E = 6.6e-19)
• PF22336: RhiE-like_linker
  • amino acids 454 to 519 (66 residues), 44.7 bits, see alignment (E = 6.5e-15)
• PF00698: Acyl_transf_1
  • amino acids 535 to 810 (276 residues), 199.2 bits, E=7.5e-62
• PF21089: PKS_DH_N
  • amino acids 900 to 995 (96 residues), 44.4 bits, 8.1e-15
• PF14765: PS-DH
  • amino acids 1021 to 1181 (161 residues), 105.1 bits, 1.7e-33
• PF08659: KR
  • amino acids 1421 to 1600 (180 residues), 191.6 bits, E=7.2e-60
• PF00106: adh_short
  • amino acids 1424 to 1543 (120 residues), 28.2 bits, 7.7e-10
• PF00550: PP-binding
  • amino acids 1703 to 1764 (62 residues), 40.5 bits, see alignment (E = 1.7e-13)
• PF00975: Thioesterase
  • amino acids 1823 to 2060 (238 residues), 105.8 bits, E=2.4e-33

Best Hits

KEGG orthology group: None (inferred from 100% identity to mag:amb1949)

Predicted SEED Role

"3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.41)" in subsystem Fatty Acid Biosynthesis FASII or mycolic acid synthesis (EC 2.3.1.41)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (48/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (15/16 steps found)
• oleate biosynthesis IV (anaerobic) (13/14 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (17/20 steps found)
• biotin biosynthesis I (13/15 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (8/9 steps found)
• fatty acid elongation -- saturated (5/5 steps found)
• superpathway of fatty acid biosynthesis initiation (5/5 steps found)
• gondoate biosynthesis (anaerobic) (4/4 steps found)
• 8-amino-7-oxononanoate biosynthesis I (9/11 steps found)
• (5Z)-dodecenoate biosynthesis I (5/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (9/12 steps found)
• palmitate biosynthesis III (21/29 steps found)
• fatty acid biosynthesis initiation (mitochondria) (3/4 steps found)
• (5Z)-dodecenoate biosynthesis II (4/6 steps found)
• stearate biosynthesis IV (4/6 steps found)
• tetradecanoate biosynthesis (mitochondria) (17/25 steps found)
• streptorubin B biosynthesis (20/34 steps found)
• mycolate biosynthesis (20/205 steps found)
• superpathway of mycolate biosynthesis (21/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.41

Sequence Analysis Tools

See Q2W5X2 at UniProt or InterPro

Protein Sequence (2076 amino acids)

>AMB_RS09835 KR domain-containing protein (Magnetospirillum magneticum AMB-1)
MRGVRKPLDIAIIGMACRFAGASDWRAYWRNLLDGVESVTFLTDAQMIEAGVDPARLRHP
DYVKAAFLLPGTENFDPQFFGYSPHEARLIDPQQRHLLEVSWEAFEDAGYPPGQDHGPVG
VYAATGSVVTNYMMNELADHPDSRGATASLLHIGNDKDFAATRVSFKLNLTGPSLNVQTA
CSTSLVLVHLACNAIQGGECAMALAAAGIVRVPQAAGYMGVKGAIFAPDGHIRTFDAEAG
GTVFSSGVAAMLFKGLDQAVADGDNIYGVIRGTALNNDGGRKASYAGTSVRGQTDVMKAA
LAQSGFDPSTIGAVECHGTGTIVGDPREFTALQRAFEGDRRQGKCPMGSTKPNIGHSEQS
AGLASLIKTALSLGEGVVPPTINYRTPNPRMKIEQSRFFVNTERLSWPEVPEHPRRALVN
GLGIGGTNGVVVMEQAPPPRPRTGAAPRPRHLVVLSGKSAGALDANAARHRAWALAHPDM
AMGDYAHTLAVGRTHFDHRFACVAASPAELAARLEGFKADPKAARKASQPRRLAFLFSGQ
GAQVAGMGRTLYEIEPTFRAELDAVAAALDPHLDRKLLDVLFADGEAIHNTGYTQPCLFA
VEVALARTLAAWGIRPEAVCGHSVGEFAACVVAGVYSLDDAARLVATRGRMMQALPAGGA
MSAVFAEEAVVAEVLAALDGKKIGIAAVNGPQGTVISGEAAEVAKAVAALEAKGIPSKAL
TVSHAFHSPLMDPALPELDAAVWAVQANAKAPALGWVSTYTGQSKKDAPDSEYWCSQARQ
AVRFADAVAALAKSGIRDFVEIGPGGALLALGRSVAGAEGLGWLPTLGKGDEHQTLLESV
GALYRAGNDPDWAAFDAPFDLRRLSLPVYAFQHERVWATKDYEPVGGRRAAVPAGTGLTG
RRLPSPLAAHQFETEFSRQRFGWLDDHRVHGAVVLPTTAGLLAALEGARAKLGEGPLEVA
GFSHGDALVLADDEARLGHLVVEANGDGAGFSLSSRGGDEDKWRTHMRGEIRRAKPGKAS
TFDAKALKRRCHLPVKADSYYAGMRSLGLAYGPSFRAIESLWRGEGEALARLRLPDLVEP
LGNGLMHPALLDACLHVYPAVIEEHGDFTQPPPEGSSAHLPLAMERFSGARSEERELWVH
ARRRKPNGHGADITVIDIEAFDSDGRPVASFDGLSVKKLPASLFKPRRADGEDGWLYVPR
WDDRPVPPVAAKASGPLTWLLLGGDGFAKPLKEALGRTGAACRLVAAKDCPTDLEGFSRL
LEGFAAEAGAGKAGILHLGAVDAPGEEAWGDEGAQRRLYDSVLGLSRALGVNRTGFLVAP
KLHLVTRNAADATGTEPPAHVLGAGMWGMGRSLALEAPALWGGLIDLEAGGASGAEAQLL
AAHLAAADDEAQVALRGGKRLAARLVRAPVPERRPAAGEGGTYLVTGGLGALGVLTAEWL
AASRKAAHLVLVSRQGDKAANAAEVLARLEALGAKVTIAKADIAKEGDVARLLGRLAKAE
PALKGIYHSAGVLDDGLVDQMDWTKFRRALAPKLDAGWWLHKHSQGLKLHDFVLYSSVLS
VIGSAGQTNYTTGNACLDALAAHRRALGLPALSVNWGAWASAGMADGLGSQGEAIWRARG
IHYIEPDLGREAFDLLFDGAAGQAVVLPADWPTFLRQFQVPPRFYAELQGGAVGGLAADI
EALKQAMAAPEPGIRRAAITDFLARQVMTTLGMTAKVESDRPLRELGLDSLMSVTLINRV
ESATGLRIPAVKLIKGPSIDELVADVWPDLAGVEVSAPAAASAAAETVVEVDAAPRPQPK
PAINAGAGKWLVTVAPRANPRYRVFCFPFAGGGSAAFQAWSATTDPAIEVIAVEPPGRLA
RISEKPVRDMDVFVDHVIDEMEETGKLDLPFALFGHCLGGLTLYEVARTLIHETETKPRH
LFCSGARCPDRVRIVGPFEKNLALHLATMPGYKPNLPPYRQADPIFAEIIRQFDMAASDQ
FLDDPELRRLMLPAVRAEFEMTSKYRYVPEKPWDIPITCFVSKGDPYVSRQDILGWGRFT
NSRMQVYMREGTHYSIFEDAAFIQRVIGRELMAPPS