Protein Info for AMB_RS09350 in Magnetospirillum magneticum AMB-1

Annotation: SufS family cysteine desulfurase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01979: cysteine desulfurase, SufS family
  • amino acids 194 to 587 (394 residues), 567 bits, E=1.1e-174
• PF00266: Aminotran_5
  • amino acids 212 to 581 (370 residues), 493 bits, E=1.2e-151
• PF00155: Aminotran_1_2
  • amino acids 252 to 440 (189 residues), 37 bits, E=4.9e-13
• PF01041: DegT_DnrJ_EryC1
  • amino acids 262 to 393 (132 residues), 22.5 bits, E=1.3e-08
• PF01212: Beta_elim_lyase
  • amino acids 298 to 433 (136 residues), 27.5 bits, E=3.8e-10

Best Hits

KEGG orthology group: K11717, cysteine desulfurase / selenocysteine lyase [EC: 2.8.1.7 4.4.1.16] (inferred from 100% identity to mag:amb1849)

Predicted SEED Role

"Cysteine desulfurase (EC 2.8.1.7), SufS subfamily" in subsystem Alanine biosynthesis (EC 2.8.1.7)

MetaCyc Pathways

• superpathway of L-alanine biosynthesis (4/4 steps found)
• L-alanine biosynthesis III (1/1 steps found)
• L-cysteine degradation IV (1/1 steps found)
• cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
• bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
• tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
• superpathway of thiamine diphosphate biosynthesis II (6/11 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (3/7 steps found)
• superpathway of thiamine diphosphate biosynthesis I (5/10 steps found)
• molybdopterin biosynthesis (2/6 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (2/6 steps found)
• tRNA-uridine 2-thiolation (thermophilic bacteria) (1/5 steps found)
• [2Fe-2S] iron-sulfur cluster biosynthesis (3/10 steps found)
• tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)
• tRNA-uridine 2-thiolation and selenation (bacteria) (2/11 steps found)

KEGG Metabolic Maps

• Selenoamino acid metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.7

Use Curated BLAST to search for 2.8.1.7 or 4.4.1.16

Sequence Analysis Tools

See Q2W672 at UniProt or InterPro

Protein Sequence (594 amino acids)

>AMB_RS09350 SufS family cysteine desulfurase (Magnetospirillum magneticum AMB-1)
MTQPLSLELPSSPSPAALPAGDWEEPSAGFAGAPDARLIAQMANALFSAQPGQSAPSFGV
APPSLPLIEAAPTGGFVPPGVPAPPSVGNVTPVPQVPQVGPLAAAPAAPPGNPAAFPPTV
LPGEPDLRGVLNQLGDVVRLVPPGHGIEPPAAAPEPPPGPSTESPPLLAIELRPDLVPDL
RGTSSRVGVFDAHAVKRDFPILNQQVHGKPLIWLDNAATTQKPRQVIERLTRYYEHENSN
IHRAAHALAARSTDAYEAAREKTRRFLNAGSTEEIVFVRGATEGINLIAKAWGERNVREG
DEIVVTWLEHHANIVPWQQLAAKVGARLRVAPVDDSGQIILEEYEKLLGPRTRLVSLTQV
SNALGTVTPAAEMTAMAHRHGALVLVDGAQAVSHMPVDVQALDCDFYVFSGHKVFAPTGI
GAVFGKKAVLEHMPPWQGGGNMIADVTFEKTLFQPAPARFEAGTGNIADAVGLGAAIDYV
EAIGMATIAAWEHQLLEYGTQGLLSIPGLRLVGTAKEKAGVLSFVIDGCRTEDIGKALDG
EGIAVRSGHHCAQPILRRFGVESTVRPSLAFYNTYEDLDALVNALRRLPSARGI