Protein Info for AMB_RS09220 in Magnetospirillum magneticum AMB-1

Annotation: 3-deoxy-8-phosphooctulonate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 283 PF00793: DAHP_synth_1" amino acids 10 to 273 (264 residues), 250.8 bits, see alignment E=6e-79 TIGR01362: 3-deoxy-8-phosphooctulonate synthase" amino acids 21 to 276 (256 residues), 389.9 bits, see alignment E=2.2e-121

Best Hits

Swiss-Prot: 70% identical to KDSA_RHOPS: 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) from Rhodopseudomonas palustris (strain BisB5)

KEGG orthology group: K01627, 2-dehydro-3-deoxyphosphooctonate aldolase (KDO 8-P synthase) [EC: 2.5.1.55] (inferred from 100% identity to mag:amb1822)

MetaCyc: 47% identical to 3-deoxy-8-phosphooctulonate synthase subunit (Arabidopsis thaliana col)
3-deoxy-8-phosphooctulonate synthase. [EC: 2.5.1.55]

Predicted SEED Role

"2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase (EC 2.5.1.55)" in subsystem KDO2-Lipid A biosynthesis (EC 2.5.1.55)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.55

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W699 at UniProt or InterPro

Protein Sequence (283 amino acids)

>AMB_RS09220 3-deoxy-8-phosphooctulonate synthase (Magnetospirillum magneticum AMB-1)
MTQTRHHVAVNDDVILGNDLPLVLIAGPCQMESRDHAMECAEALKIMAAEAGIALIYKSS
FDKANRTSLAGQRGVGLDKALPIFAEIKAKLGLPVLTDVHTEEQCVIAAKVADVLQIPAF
LCRQTDLLVAAGRTGAVINVKKGQFLAPWDMANVAAKIESTGNSRVLLTERGASFGYNTL
VTDMRGLPIMARTGWPVIMDATHAVQAPGGQGNSSGGDRRFAPVLARAAVAIGVAGVFVE
CHPDPDHAPSDGPNMIAMKDMPALVEVLMKLDATAKSFPVSID