Protein Info for AMB_RS08310 in Magnetospirillum magneticum AMB-1

Annotation: hydrogenase accessory protein HypB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 312 TIGR00073: hydrogenase accessory protein HypB" amino acids 77 to 287 (211 residues), 292.8 bits, see alignment E=6.8e-92 PF02492: cobW" amino acids 107 to 265 (159 residues), 84.2 bits, see alignment E=4.6e-28

Best Hits

Swiss-Prot: 63% identical to HYPB_RHILV: Hydrogenase maturation factor HypB (hypB) from Rhizobium leguminosarum bv. viciae

KEGG orthology group: K04652, hydrogenase nickel incorporation protein HypB (inferred from 100% identity to mag:amb1642)

Predicted SEED Role

"[NiFe] hydrogenase nickel incorporation-associated protein HypB" in subsystem NiFe hydrogenase maturation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W6S9 at UniProt or InterPro

Protein Sequence (312 amino acids)

>AMB_RS08310 hydrogenase accessory protein HypB (Magnetospirillum magneticum AMB-1)
MCTVCGCGDAKVDGHAHSPHSHDHDHEHHHGHSHDHSHDHPHGHDHHHHDHDSDKDIHYG
RGIAGVHVPGMSQKRIVQIEKDILSHNDDFAGRNRAWLAEHGVFALNLVSSPGSGKTTLL
CRTAEALKGRYPLAVIEGDQQTSFDADRIRATGIPAIQVNTGKGCHLDAHMVESAMAKLN
VADDSVLFIENVGNLVCPAAFDLGEAYKVAILSVTEGEDKPLKYPDMFHAASMMILNKTD
LLPHTDFDVERCIGFARRVNPDIVVLQLSARTGEGFDEWLAWINEGRKRMVTERIRRLEA
KLAAAKASLEGK