Protein Info for AMB_RS07995 in Magnetospirillum magneticum AMB-1

Annotation: Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to GATA_MAGSA: Glutamyl-tRNA(Gln) amidotransferase subunit A (gatA) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K02433, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A [EC: 6.3.5.6 6.3.5.7] (inferred from 100% identity to mag:amb1585)

Predicted SEED Role

"Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)

MetaCyc Pathways

L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
ammonia assimilation cycle III (3/3 steps found)
L-glutamate biosynthesis I (2/2 steps found)
L-glutamine degradation I (1/1 steps found)
L-citrulline biosynthesis (6/8 steps found)
superpathway of L-citrulline metabolism (8/12 steps found)
L-glutamate and L-glutamine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7

Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W6Y6 at UniProt or InterPro

Protein Sequence (490 amino acids)

>AMB_RS07995 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit A (Magnetospirillum magneticum AMB-1)
MSKLTDLTMAEARDGLAKGAFTAVELTDAHIKATEAQRHLNAFIVETPDLALEAAKASDA
RRQAGTAGSMDGLPIGIKDLFCTEGVQTTAASHILEGFKPPYESTVSGKLKAAGAISLGK
LNLDEFAMGSSNQTSYFGAVENPWKKTSDPKAKLVPGGSSGGSAAAVAARMVLGATGTDT
GGSIRQPAAFCGITGIKPTYGRCSRFGIVAFASSLDQAGPMARTVRDCAIMLGAMAGHDP
KDSTSVNMAVPDFERALTGDIRGLKVGIPKEYRPDGLSDEVAKVWDRGIEWLKAAGATPV
EISLPHTKYALATYYIIAPAECSSNLARYDGLRYGLRVPGKTLDDMYKKSRAAGFGAEVR
RRILIGTYVLSAGYYDAYYAKAQKVRRLIAEDFRKAFETVDVILTPTAPSAAFGMGEGTD
DPVTMWLNDVFTIPTSMAGLPGLSLPAGLSADGLPLGLQLIGRPFDEETVFRVAGVMETA
ANFTAKPEGV