Protein Info for AMB_RS07835 in Magnetospirillum magneticum AMB-1

Annotation: acyl-CoA synthetase AMP-fatty acid ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 17 to 362 (346 residues), 244.3 bits, E=1.9e-76
• PF13193: AMP-binding_C
  • amino acids 412 to 489 (78 residues), 57.5 bits, E=2.2e-19

Best Hits

KEGG orthology group: None (inferred from 100% identity to mag:amb1551)

Predicted SEED Role

"Benzoate-CoA ligase (EC 6.2.1.25)" in subsystem Benzoate transport and degradation cluster (EC 6.2.1.25)

MetaCyc Pathways

• benzoate degradation II (aerobic and anaerobic) (1/1 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (7/17 steps found)
• anaerobic aromatic compound degradation (Thauera aromatica) (11/27 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.25

Use Curated BLAST to search for 6.2.1.25

Sequence Analysis Tools

See Q2W720 at UniProt or InterPro

Protein Sequence (500 amino acids)

>AMB_RS07835 acyl-CoA synthetase AMP-fatty acid ligase (Magnetospirillum magneticum AMB-1)
MNAADEILGPSLGSGRGESLAIICGEQTLTYDQLNRLACRFGNAMLAAGVQRQQPVLLLL
DDGPELVAAYLGAMKAGLVAVALNTRLSPKDLSHALGDSGAPLLLAETALKDLAAESLAL
AHASARMVTTDELDAFLEGASDQLVSADMGPEDMALWMYTSGTTGQPKGAVHVHGSIPLG
ERHVRENLGLLPGDRIFSTSKLFFAYPLGHCLIGALRCGGTLVLHRGWPDATAAAEVIAR
TRPKLVLSVPSLYRIMLKDGVGSSPAFREVRTWVSAGENLPADLCRRWMEETGGLMLEGI
GATEALFLFIASTPTAMKPGACGRPLPWAEAQLRSPSGEVIAAPDTPGDLWVRMDSLFRR
YHNRPDVTQRVLKDGWWKTGDVFSFDAEGWWSPQGRSDDMIKVSGQWVSPSEVEEAALMV
PGVADAVAVGIPNEDGLVRLVLYAVAEAGEHEPLLETRIVETLRSKLAIYKCPRNVRFLE
TIPRTATGKVQRFKLREAGA