Protein Info for AMB_RS07095 in Magnetospirillum magneticum AMB-1

Annotation: protein nirF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 424 signal peptide" amino acids 1 to 59 (59 residues), see Phobius details PF02239: Cytochrom_D1" amino acids 59 to 418 (360 residues), 351.2 bits, see alignment E=5.1e-109

Best Hits

Swiss-Prot: 59% identical to NIRF_PSEST: Protein NirF (nirF) from Pseudomonas stutzeri

KEGG orthology group: None (inferred from 100% identity to mag:amb1402)

MetaCyc: 60% identical to heme d1 dehydrogenase (Paracoccus pantotrophus)
1.1.1.-

Predicted SEED Role

"Heme d1 biosynthesis protein NirF" in subsystem Dissimilatory nitrite reductase

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W7G9 at UniProt or InterPro

Protein Sequence (424 amino acids)

>AMB_RS07095 protein nirF (Magnetospirillum magneticum AMB-1)
MKRLACHPERKRGISSWRTFSGLASMAQAEIPRLRLGMTRMAALLGLGLLSACSPALRGT
GDLGLVVERADGRLMVVENSGNTKLASVAGLGDLSHAALVYSRDQRFAFVFGRDGGLSKV
DILTGAIVGRVMQSGNSIGGAISQDGTLVAVANYTPGGIKVFDAATLEPVADIPAIGGDG
KPSKVIGIADLTGKRFIYTLYDAGEIWIADLADPKAPKLTKYTNIGKLPYDALITGDGRW
YLAGLFGEDGIALLDLWNPDKGVRRILDHYGKGKEALPVYKMPHLRGWAAAGDEIWLPAI
GHHQVLVVDKGRWTEKARIDVAGQPVFAMARPDGRQVWVNFAVPDYSKVQVIDTQTKKVI
KTLEPGLAILHMEFTPKGERVWLSVRDSDKVVVYDTETLEPVAELPAARPSGIFFTNRAH
KTGL