Protein Info for AMB_RS06760 in Magnetospirillum magneticum AMB-1

Annotation: fumarate hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF05681: Fumerase
  • amino acids 48 to 322 (275 residues), 271 bits, E=1.1e-84
• TIGR00722: hydrolyase, tartrate alpha subunit/fumarate domain protein, Fe-S type
  • amino acids 65 to 321 (257 residues), 100.7 bits, E=8.1e-33
• PF05683: Fumerase_C
  • amino acids 330 to 535 (206 residues), 288.1 bits, E=3.1e-90
• TIGR00723: hydrolyase, tartrate beta subunit/fumarate domain protein, Fe-S type
  • amino acids 371 to 535 (165 residues), 186.4 bits, E=4.1e-59

Best Hits

Swiss-Prot: 67% identical to FUMA_SALTY: Fumarate hydratase class I, aerobic (fumA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K01676, fumarate hydratase, class I [EC: 4.2.1.2] (inferred from 100% identity to mag:amb1331)

MetaCyc: 67% identical to fumarase A (Escherichia coli K-12 substr. MG1655)
Oxaloacetate tautomerase. [EC: 5.3.2.2]; Fumarate hydratase. [EC: 5.3.2.2, 4.2.1.2]

Predicted SEED Role

"Fumarate hydratase class I (EC 4.2.1.2)" in subsystem TCA Cycle (EC 4.2.1.2)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (20/22 steps found)
• TCA cycle V (2-oxoglutarate synthase) (9/9 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle III (animals) (9/10 steps found)
• TCA cycle VIII (Chlamydia) (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• TCA cycle VII (acetate-producers) (8/9 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (20/26 steps found)
• reductive TCA cycle I (9/11 steps found)
• incomplete reductive TCA cycle (6/7 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• superpathway of glyoxylate bypass and TCA (9/12 steps found)
• methylaspartate cycle (14/19 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• reductive TCA cycle II (8/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (9/14 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
• pyruvate fermentation to propanoate I (3/7 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• mixed acid fermentation (9/16 steps found)
• L-glutamate degradation VIII (to propanoate) (3/11 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.2

Use Curated BLAST to search for 4.2.1.2 or 5.3.2.2

Sequence Analysis Tools

See Q2W7P0 at UniProt or InterPro

Protein Sequence (538 amino acids)

>AMB_RS06760 fumarate hydratase (Magnetospirillum magneticum AMB-1)
MSDFAFHELFPLSHDDTPYRKITAEGVGTASFNGQSVVTVAPEAITALTKEAIKDTSHLL
RPAHLQQLRNILDDAGSSPNDRFVAYDLLKNACIASGGVLPMCQDTGTAIVMGKKGQRVW
TGGNDEAAISKGVQEAYTELNLRYSQTAPLDMYEEVNTGTNLPAQIDLYATEGEAYKFMF
MAKGGGSANKTFLYQQTKALLNPKSLLSFLDAQIRTLGTSACPPYHLAIVIGGLSAEMCL
KTVKLASAKYLDSLPTAGNKFGQAFRDLELEQIVLKMTQDMGIGAQFGGKYFCHDVRVIR
LPRHGASCPVAIGVSCSADRQILGKITKDGVYVEAMEKDPAKYLPDVDPSKLAGEVVKID
LNRPMDEIRKTLSQYPVKTRVSLTGPMIVARDIAHAKLKERLDRGEGLPEYFKQMGVYYA
GPAKTPSNFASGSFGPTTAGRMDSYVDQFQAAGGSMLMIAKGNRSKAVTEACKKHGGFYL
GSIGGAAARLAQDCIKKVEVIEYPELGMEAIWRIEVVDFPAFIVVDDKGNDFFAEFAH