Protein Info for AMB_RS06275 in Magnetospirillum magneticum AMB-1

Annotation: cysteine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 459 TIGR00435: cysteine--tRNA ligase" amino acids 3 to 458 (456 residues), 512.5 bits, see alignment E=6.5e-158 PF01406: tRNA-synt_1e" amino acids 16 to 313 (298 residues), 406.7 bits, see alignment E=1.7e-125 PF00133: tRNA-synt_1" amino acids 222 to 301 (80 residues), 25.8 bits, see alignment E=1.1e-09 PF09190: DALR_2" amino acids 342 to 400 (59 residues), 57.9 bits, see alignment E=2.9e-19

Best Hits

Swiss-Prot: 100% identical to SYC_MAGSA: Cysteine--tRNA ligase (cysS) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K01883, cysteinyl-tRNA synthetase [EC: 6.1.1.16] (inferred from 100% identity to mag:amb1236)

Predicted SEED Role

"Cysteinyl-tRNA synthetase (EC 6.1.1.16)" in subsystem Conserved gene cluster possibly involved in RNA metabolism (EC 6.1.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W7Y5 at UniProt or InterPro

Protein Sequence (459 amino acids)

>AMB_RS06275 cysteine--tRNA ligase (Magnetospirillum magneticum AMB-1)
MPLVLYNTLTRTKDVFEPLVPGHVGMYVCGPTVYDRAHIGNARPVIVFDVLYRLLKTLYP
SVRYVRNITDVDDKINARAKESGEPISAITERTTAMFHEDIGALNCLLPDVEPRATAHIT
QMIAMIERLIAKGFAYAAEGHVLFAVGAMADYGALSRRSSDEMIAGARVEVAPYKKDPGD
FVLWKPSSDDLPGWDSPWGRGRPGWHIECSAMSLQHLGETFDIHGGGQDLVFPHHENEIA
QSTCANGKPFARFWLHNGWLMVEGEKMSKSLGNFFTVRDLLDQAPGEAIRLAMLTTHYHQ
PFDWTADGLKQAKATLDRLYTALRGVADIDANGYEGEAPLEVLAALKDDLNTPLAISHLH
ELAGALNKATGLEDKARRKGALLASGHLLGLLYQDPEAWFKGTGAADGPSDAEIEAAITA
RAEARKSRNFAEADRIRQDLAARGVVLEDGAGGTTWKRG