Protein Info for AMB_RS06270 in Magnetospirillum magneticum AMB-1

Annotation: glutamate--tRNA ligase 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00749: tRNA-synt_1c
  • amino acids 5 to 292 (288 residues), 225.6 bits, E=7.5e-71
• TIGR00464: glutamate--tRNA ligase
  • amino acids 6 to 324 (319 residues), 291.8 bits, E=5.2e-91
• PF19269: Anticodon_2
  • amino acids 382 to 439 (58 residues), 37.3 bits, 3.7e-13

Best Hits

Swiss-Prot: 100% identical to SYE1_MAGSA: Glutamate--tRNA ligase 1 (gltX1) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 100% identity to mag:amb1235)

Predicted SEED Role

"Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24)" in subsystem tRNA aminoacylation, Glu and Gln (EC 6.1.1.24)

MetaCyc Pathways

• adenosylcobalamin biosynthesis II (aerobic) (28/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
• tRNA charging (18/21 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• tetrapyrrole biosynthesis I (from glutamate) (5/6 steps found)
• superpathway of heme b biosynthesis from glutamate (7/10 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Glutamate metabolism
• Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.17

Use Curated BLAST to search for 6.1.1.17 or 6.1.1.24

Sequence Analysis Tools

See Q2W7Y6 at UniProt or InterPro

Protein Sequence (444 amino acids)

>AMB_RS06270 glutamate--tRNA ligase 1 (Magnetospirillum magneticum AMB-1)
MTSPVVRFAPSPTGLLHVGNARVALLNRLFAKAHGGSFILRLDDTDLERSRPEFAEAILD
DLRWLGLDWDRLERQSARLDAYAAAAERLKVSGRVYACYETPDELELRRKVQLGRGLPPV
YDRAALKLAPDEIARLEAEGRRPHWRFKLELEDVRWDDCVRGPSHYHGGNLSDPVLIRGD
GSFLYTLPSVVDDIEFGVTHIIRGEDHVTNTAPQIQLFQALGAVPPAFAHLPLLTGATGE
GLSKRLGSGSLKDLRATGIHPMALNSLLAKLGSSDAIDIRHTLAELEAEFAWDKFGRGTP
KFDSAELERLDARLMHTASFAEVRDRLGIDGADEEFWLAVRPNLNHLAEAEAWWAICRQD
LTPVIEDGEFTRAAAALLPDGAWDHATWGAWTETVKQATGRKGKALFHPLRLALTGRENG
PELKTLLPLIGHDRARARLMGEIA