Protein Info for AMB_RS05685 in Magnetospirillum magneticum AMB-1

Annotation: D-alanyl-D-alanine carboxypeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 435 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details PF00768: Peptidase_S11" amino acids 33 to 253 (221 residues), 190.3 bits, see alignment E=5.8e-60 PF13354: Beta-lactamase2" amino acids 40 to 186 (147 residues), 46 bits, see alignment E=6.7e-16 PF05036: SPOR" amino acids 353 to 428 (76 residues), 30.8 bits, see alignment E=4.7e-11

Best Hits

KEGG orthology group: K01286, D-alanyl-D-alanine carboxypeptidase [EC: 3.4.16.4] (inferred from 100% identity to mag:amb1118)

Predicted SEED Role

"D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.16.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4

Use Curated BLAST to search for 3.4.16.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W8A3 at UniProt or InterPro

Protein Sequence (435 amino acids)

>AMB_RS05685 D-alanyl-D-alanine carboxypeptidase (Magnetospirillum magneticum AMB-1)
MHLSRTGFLNRLRLIAVALGVAIIAVAQPAEAGRYASIVIDAENGSVLHAANPDTRSYPA
SLTKVMTLFLLFDELDSGRIRLDSKFTTSAHAAAQSPSKLGLEPGEKISVESLILALVTK
SANDAAVVAAEGVGGTETRFAHMMTQKAHALGMRNTVYRNASGLPDTGQVSTVRDQATLA
RALIRSHPGYYKYFSTRQFVYDGQPINTHNRLMLRYQGADGIKTGYIHASGFNLISSAKR
GDKRIIGVVFGGSTASSRDNHMGQLLDKGFAKLRRGGGVEMAAAETETEGNDDLPDLDDL
EAAAQAAKAPAKAPAKAVAKAKPAPAKKVVLRAPARAADDDDDDDDAVGDADPSGWAIQV
GAFSEYRPAHKAASDAAKKLGGLVSKASIDIDKAGKGAKALYRARISGFTEDQARAACKR
LGKAGKSCKTVNPNA