Protein Info for AMB_RS05665 in Magnetospirillum magneticum AMB-1

Annotation: NADH ubiquinone oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01058: Oxidored_q6
  • amino acids 85 to 246 (162 residues), 81.3 bits, E=5.3e-27
• PF14720: NiFe_hyd_SSU_C
  • amino acids 264 to 341 (78 residues), 81.4 bits, E=5.1e-27

Best Hits

KEGG orthology group: K05927, quinone-reactive Ni/Fe-hydrogenase small subunit [EC: 1.12.5.1] (inferred from 100% identity to mag:amb1115)

Predicted SEED Role

"Uptake hydrogenase small subunit precursor (EC 1.12.99.6)" in subsystem Hydrogenases or Membrane-bound Ni, Fe-hydrogenase (EC 1.12.99.6)

MetaCyc Pathways

• hydrogen oxidation I (aerobic) (1/1 steps found)
• hydrogen to dimethyl sulfoxide electron transfer (1/2 steps found)
• hydrogen to fumarate electron transfer (1/2 steps found)
• hydrogen to trimethylamine N-oxide electron transfer (1/2 steps found)

KEGG Metabolic Maps

• Trinitrotoluene degradation

Isozymes

Compare fitness of predicted isozymes for: 1.12.5.1, 1.12.99.6

Use Curated BLAST to search for 1.12.5.1 or 1.12.99.6

Sequence Analysis Tools

See Q2W8A6 at UniProt or InterPro

Protein Sequence (384 amino acids)

>AMB_RS05665 NADH ubiquinone oxidoreductase (Magnetospirillum magneticum AMB-1)
MSRFISLSSTNKILAMIAPGQRRPINWKGTYSKKLFDRPIACNYNHPRQIAINFEPYNKY
MMKEVAGGRRPGMNLNLYWLQCGGCGGDTMSFLNSETPDILQLFSGLGIDVVYHPSLSNT
SQAEQHSQLEVISSGEMPLDILVVEGAVLRGPAGTGMYDTKGRRPKKDIVAALARQARFV
VAAGTCAAFGGFGARTEIEATGLQFHRSEPGGFLGADFRAKSGLPVLNLSGCPCHPQVLS
SVLTALATGAELPLAPLNRPLPWYGMMVHQGCTRNEYHEYRVEEDNFGEKGCLFFHMGCH
GPLVTGPCNKLLWNQRSSKTRAGVPCFGCTSPEFPQDHAFFETRNIEGIPLDLPNGINRA
HYMVYKVMAAAAAPERLRQRRTDI