Protein Info for AMB_RS05185 in Magnetospirillum magneticum AMB-1

Annotation: heavy metal translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 716 transmembrane" amino acids 75 to 94 (20 residues), see Phobius details amino acids 100 to 120 (21 residues), see Phobius details amino acids 132 to 149 (18 residues), see Phobius details amino acids 154 to 172 (19 residues), see Phobius details amino acids 302 to 324 (23 residues), see Phobius details amino acids 333 to 360 (28 residues), see Phobius details amino acids 646 to 663 (18 residues), see Phobius details amino acids 669 to 689 (21 residues), see Phobius details TIGR01525: heavy metal translocating P-type ATPase" amino acids 134 to 689 (556 residues), 561.6 bits, see alignment E=3.6e-172 TIGR01494: HAD ATPase, P-type, family IC" amino acids 159 to 663 (505 residues), 244.1 bits, see alignment E=3.4e-76 PF00122: E1-E2_ATPase" amino acids 185 to 365 (181 residues), 161.7 bits, see alignment E=1.4e-51 PF00702: Hydrolase" amino acids 383 to 598 (216 residues), 109.1 bits, see alignment E=3.9e-35

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 100% identity to mag:amb1012)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W8K9 at UniProt or InterPro

Protein Sequence (716 amino acids)

>AMB_RS05185 heavy metal translocating P-type ATPase (Magnetospirillum magneticum AMB-1)
MCCVEEVRTLKAAVGPLAGGDERLSFDLLNGKMIVRADIDADAVIAAVSRTGMGAEPWER
TSVAERDKANSSRRLLQAVLAGLSGGAALLGLILDQAVKVPLPAQIAEAVAVAVGLWMVL
PKALHAVRSLRPDMNLLMSVAVFGAMALGDWMEAATVSALFALSLALESWSAERARRAIA
SLMDLTPPMARVKGTDGAESLAAPEDVPVGAVFVVLPGERIPLDGRVLVGESMVDQAPIT
GESVPVLKSVGTEVFAGTINADGVLEVQNLKPAGETTLAKVARLVEEAQGKRSKVERWVD
RFAAIYTPVVLAGAVAVAVLPPLALGLDWSEWIYRSLVLLVISCPCALVISTPLTVVAAM
ASAARAGVLVKGGEYLETASRLSAIAFDKTGTVTSGRPGVERVMALDGGDEGRILRLAAA
LEARSTHPLGRAILDHAAAGGIAPTPAESVQSLPGKGMCGVVDGEAVWLGSHRYAMERGA
ETPAVREAALALAEGGRSVVAVGDAAGVRGLIALSDPIRPEAATALAGLRRAGVGKLIML
TGDNAATGERVAAALGFDLVLAELLPEDKSEAMDDLARQFGTVAMVGDGVNDAPAMARSS
LGIAMGAAGTDTAIETSDLALMSDDLTRLAWLIGHSRRMMTVIRQNIGFAITLKAVFMVL
AILDIATLWGAIAADMGAALLVAFNGLRLLKAGVTQRSEDGMGLSSSSNTLKQRLA