Protein Info for AMB_RS04565 in Magnetospirillum magneticum AMB-1

Annotation: PAS domain-containing sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 655 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 175 to 181 (7 residues), see Phobius details amino acids 251 to 271 (21 residues), see Phobius details TIGR00229: PAS domain S-box protein" amino acids 289 to 411 (123 residues), 90 bits, see alignment E=6.5e-30 PF13188: PAS_8" amino acids 291 to 343 (53 residues), 26.1 bits, see alignment 2.2e-09 PF00989: PAS" amino acids 291 to 403 (113 residues), 62.7 bits, see alignment E=1.1e-20 PF08448: PAS_4" amino acids 299 to 408 (110 residues), 43.3 bits, see alignment E=1.3e-14 PF13426: PAS_9" amino acids 307 to 405 (99 residues), 48.1 bits, see alignment E=4.2e-16 PF08447: PAS_3" amino acids 313 to 396 (84 residues), 29.1 bits, see alignment E=3.4e-10 PF00512: HisKA" amino acids 429 to 496 (68 residues), 34 bits, see alignment E=8.5e-12 PF02518: HATPase_c" amino acids 545 to 652 (108 residues), 91.2 bits, see alignment E=2e-29

Best Hits

KEGG orthology group: K11711, two-component system, LuxR family, sensor histidine kinase DctS [EC: 2.7.13.3] (inferred from 100% identity to mag:amb0896)

Predicted SEED Role

"two-component hybrid sensor and regulator"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W8X5 at UniProt or InterPro

Protein Sequence (655 amino acids)

>AMB_RS04565 PAS domain-containing sensor histidine kinase (Magnetospirillum magneticum AMB-1)
MNAPRSLRDRQAIQAMPVIAMGLVVMLLGVLLWLLHRNEVEEERRALIQDILWVEQNLYF
HLSSGVERLGQLAEMAGRDGPWSDEFVLQGKALVTAAPEIERVIVRDGIGAVVQSLPPVE
SGAALGGDSEWANTFALSRSSGRPVYGPPYTVEGKGARFEAQVPIFRGGRFAGTVAGVFS
LDAMLANRVPWWFAQAYRLEIQDGNGTLLAAKSQLSPAEPGASYTLRFEPPGHGLELVAI
SYHSDPQLLRNVLAAAIFILSILSVSSLWALRRHVRRRLAAEQALRTEHSFRKAMEDSLT
VGMRARDLEGRITYVNPAFCHMVGWTADELVGTVPPMLYWVPEDLEHTYELHRAVMRGEA
PAEGFELVFRRKNGERFSALIYEAPLIDSDGRHTGWMASVLDITERKRAEELSRQHQEKL
QHTARLITMGEMASTLAHELNQPLSAIASYATGCLNRLSAGDARPEELVPPLTKLGAQAQ
RAGQIIRRIHDFVRKSEPLVVPCLLDEIIEGVVGFLEPDARKRGIRIDLDLSLTPGAARP
RVEADHILIEQVILNLVRNAMEAMGHTPRPQRRLTISLQCADGQARIRVADRGTGIPPEI
AANLFSPFFTTKETGMGMGLNICRSIVEAHRGHLWFEENPGGGSVFLFTLPEMAG