Protein Info for AMB_RS04015 in Magnetospirillum magneticum AMB-1

Annotation: phosphoenolpyruvate carboxykinase (GTP)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 617 PF17297: PEPCK_N" amino acids 21 to 241 (221 residues), 345.1 bits, see alignment E=1.7e-107 PF00821: PEPCK_GTP" amino acids 245 to 601 (357 residues), 541 bits, see alignment E=1.1e-166

Best Hits

Swiss-Prot: 69% identical to PCKG_GEOSL: Phosphoenolpyruvate carboxykinase [GTP] (pckG) from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)

KEGG orthology group: K01596, phosphoenolpyruvate carboxykinase (GTP) [EC: 4.1.1.32] (inferred from 100% identity to mag:amb0785)

Predicted SEED Role

"Phosphoenolpyruvate carboxykinase [GTP] (EC 4.1.1.32)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 4.1.1.32)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.32

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W986 at UniProt or InterPro

Protein Sequence (617 amino acids)

>AMB_RS04015 phosphoenolpyruvate carboxykinase (GTP) (Magnetospirillum magneticum AMB-1)
MEMTKSGIPGGTTMNAKLLAWVDEMAHLCRPSGIHICDGSQKEYDALCEMMVAGGTMIKL
NPAKRPGSYLCRSDPRDVARVEDRTFICSHNKNDAGPTNNWVEPREMKARMTKLYDGCMK
GRTMYVVPFSMGPLGSDIAHIGVQITDSAYAAANMRIMTRMGQPVLDILGTDGDFVQCLH
SVGKPLAEGEKDVAWPCNPDNITIAHFPEDRQIWSFGSGYGGNALLGKKCFALRIASAMA
RDEGWLAEHMLIVGVESPKGEKTYVAAAFPSACGKTNFAMLVPPKGFDGWKIYTVGDDIA
WIKPGPDGRFYAINPEAGFFGVAPGTGATTNPSAIAACAKNSIFTNVAMTDDGDVWWEGL
TDEAPAHLIDWKGNDWTPESKDPAAHPNSRFTAPVAQCPSVDPDWENPAGVPISAFIVGG
RLSKNAPLVVQSFNWAHGVYLGATMGSEATAAAVGQAAIRRDPFAMLPFCGYNMADYFNH
WLNMGRAVKNPPPIFRVNWFRRDENNKFMWPGYGQNMRVLKWIVDRVGGKAKAVESPIGL
VPQYEDMDWNGLDYPRDKFKDLMRIDRDAGVADARSQEELFDKFFDRVPKEMLFERELLK
SRLWRAPAVWEMASEKI