Protein Info for AMB_RS03985 in Magnetospirillum magneticum AMB-1

Annotation: bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 488 TIGR02198: bifunctional protein RfaE, domain I" amino acids 12 to 323 (312 residues), 378.1 bits, see alignment E=4.7e-117 PF00294: PfkB" amino acids 17 to 313 (297 residues), 139.5 bits, see alignment E=2.4e-44 PF08543: Phos_pyr_kin" amino acids 197 to 299 (103 residues), 46.5 bits, see alignment E=5e-16 TIGR02199: bifunctional protein RfaE, domain II" amino acids 345 to 486 (142 residues), 205.4 bits, see alignment E=5.1e-65 TIGR00125: cytidyltransferase-like domain" amino acids 356 to 420 (65 residues), 48.4 bits, see alignment E=1.2e-16 PF01467: CTP_transf_like" amino acids 358 to 453 (96 residues), 58.8 bits, see alignment E=1e-19

Best Hits

Swiss-Prot: 100% identical to HLDE_MAGSA: Bifunctional protein HldE (hldE) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K03272, D-beta-D-heptose 7-phosphate kinase / D-beta-D-heptose 1-phosphate adenosyltransferase [EC: 2.7.1.- 2.7.7.-] (inferred from 100% identity to mag:amb0778)

Predicted SEED Role

"ADP-heptose synthase (EC 2.7.-.-) / D-glycero-beta-D-manno-heptose 7-phosphate kinase" in subsystem LOS core oligosaccharide biosynthesis (EC 2.7.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.-.-, 2.7.1.-, 2.7.7.-

Use Curated BLAST to search for 2.7.-.- or 2.7.1.- or 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W993 at UniProt or InterPro

Protein Sequence (488 amino acids)

>AMB_RS03985 bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (Magnetospirillum magneticum AMB-1)
MTELSALVERVEKLRGTMVLCVGDAMLDRFVYGSVERISPEAPIPVLCIERETAMLGGAG
NVVRNLVAVGAEPAFVSVVGDDTAGREVTRLVGEHGEIDPCIVVEPGRQTTIKTRFFASH
QQLLRADRESRSPVGEAIRAQLLTRIERLLPKAGVMVLSDYGKGVLAEPIAIELIRRAKA
AGKQVIVDPKGTDYTIYAGATVVTPNRKELHEATGQAVDSDEQVVAAARQLIDSCGFEAV
LVTRSQDGMTLVRADGQIDHLPAEAREVFDVSGAGDTVVATLAAALASGATLPEAAHLAN
VAAGIVVGKVGTAVAYGDELVVALHREDLTLGEAKIVPVTAAAEVVDRWRRKGQKVGFTN
GCFDLLHPGHVSILAQAKGACDKLVVGLNSDASVQRLKGPTRPVQSEASRATVLSSLATV
DLVVIFGEDTPLEVIGTLKPDVLVKGADYTIDKVVGADLVQSWGGKVVLAELVNGQSTTN
TIKKMNGN