Protein Info for AMB_RS03940 in Magnetospirillum magneticum AMB-1

Annotation: glycine dehydrogenase (aminomethyl-transferring)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 499 PF00266: Aminotran_5" amino acids 173 to 287 (115 residues), 38.8 bits, see alignment E=5.9e-14 PF21478: GcvP2_C" amino acids 362 to 458 (97 residues), 69.7 bits, see alignment E=2.2e-23

Best Hits

Swiss-Prot: 70% identical to GCSPB_CAUVC: Probable glycine dehydrogenase (decarboxylating) subunit 2 (gcvPB) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K00283, glycine dehydrogenase subunit 2 [EC: 1.4.4.2] (inferred from 100% identity to mag:amb0769)

Predicted SEED Role

"Glycine dehydrogenase [decarboxylating] (glycine cleavage system P2 protein) (EC 1.4.4.2)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 1.4.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.4.2

Use Curated BLAST to search for 1.4.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W9A2 at UniProt or InterPro

Protein Sequence (499 amino acids)

>AMB_RS03940 glycine dehydrogenase (aminomethyl-transferring) (Magnetospirillum magneticum AMB-1)
MAEINTISGNRALQIEEKLIFELGNPGSVAVDLPEPAPFDLERLGDAPRRGRVALPDLSE
PQVVRHYTRLSQKNYGIDTGFYPLGSCTMKHNPRLSEKVARLPGLADLHPLQPQKTVQGA
LEVIDTLAHWLKALTGMPAVAMSPAAGAHGEWCGLMAIRSAHEDKGEGHRKRVLVPESAH
GTNPASAAMCGYTVDPIPALENGRVDLAALKAKLGPDVACLMLTNPNTCGLFETEIVEIA
AAVHGAGAYFYCDGANFNAIVGRVKIADLGIDAMHINLHKTFATPHGGGGPGAGPTVLSA
ALAAFVPVPYVVHGASGLELVEGKRDGAKPFGRVKGFHGQFGVFVRALAYIQSMGSDGLR
QASSDAVLNANYLLASLKDELSASFDGPCMHEALFDDRFLKDTGVTTLDFAKAMIDEGYH
PMTMYFPLVVHGALLMEPTETESKDTLDQFIAVVKGLAAKAKAGGVEDFKAAPRLTPRRR
LDETLAARSPVLRWKTAAE