Protein Info for AMB_RS03840 in Magnetospirillum magneticum AMB-1

Annotation: guanylate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03263: guanylate kinase
  • amino acids 13 to 191 (179 residues), 234 bits, E=4.6e-74
• PF00625: Guanylate_kin
  • amino acids 14 to 192 (179 residues), 180.6 bits, E=2.5e-57
• PF13671: AAA_33
  • amino acids 16 to 156 (141 residues), 28.9 bits, E=1.3e-10

Best Hits

Swiss-Prot: 100% identical to KGUA_MAGSA: Guanylate kinase (gmk) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K00942, guanylate kinase [EC: 2.7.4.8] (inferred from 100% identity to mag:amb0748)

MetaCyc: 52% identical to guanylate kinase (Escherichia coli K-12 substr. MG1655)
Guanylate kinase. [EC: 2.7.4.8]; T(2)-induced deoxynucleotide kinase. [EC: 2.7.4.8, 2.7.4.12, 2.7.4.13]

Predicted SEED Role

"Guanylate kinase (EC 2.7.4.8)" in subsystem MLST or Purine conversions (EC 2.7.4.8)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (7/8 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
• guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
• pyrimidine deoxyribonucleotide phosphorylation (4/4 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• superpathway of purine nucleotide salvage (11/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (5/7 steps found)
• purine deoxyribonucleosides salvage (4/6 steps found)
• dZTP biosynthesis (3/5 steps found)
• superpathway of pyrimidine deoxyribonucleoside salvage (5/9 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.4.12 or 2.7.4.13 or 2.7.4.8

Sequence Analysis Tools

See Q2W9C3 at UniProt or InterPro

Protein Sequence (214 amino acids)

>AMB_RS03840 guanylate kinase (Magnetospirillum magneticum AMB-1)
MTDTLPTVSRRGLMLVMSSPSGAGKTTISRALLERDPAIGMSVSATTRAPRPGEVDGKDY
HFVTVEKFHEMVEKREFLEHARVFDNFYGTPRGPVDEILRSGRDVLFDIDWQGTQQMAQN
ARADLVSVFVLPPSVEELERRLRGRAQDSDEVVRKRMAKAGDEMSHWPEYDYIVVNIDLD
KSIAAVQAILAAERLKRERQVGLPDFVTQLRGGE