Protein Info for AMB_RS03290 in Magnetospirillum magneticum AMB-1

Annotation: DNA topoisomerase (ATP-hydrolyzing) subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 816 TIGR01059: DNA gyrase, B subunit" amino acids 21 to 816 (796 residues), 948.7 bits, see alignment E=7.9e-290 PF02518: HATPase_c" amino acids 48 to 163 (116 residues), 58.4 bits, see alignment E=2.4e-19 PF00204: DNA_gyraseB" amino acids 239 to 407 (169 residues), 185.3 bits, see alignment E=1.7e-58 PF01751: Toprim" amino acids 436 to 547 (112 residues), 65.4 bits, see alignment E=1.1e-21 PF21249: GyrB_hook" amino acids 550 to 574 (25 residues), 25.2 bits, see alignment (E = 2.5e-09) PF00986: DNA_gyraseB_C" amino acids 744 to 805 (62 residues), 101.7 bits, see alignment 4.8e-33

Best Hits

KEGG orthology group: K02470, DNA gyrase subunit B [EC: 5.99.1.3] (inferred from 100% identity to mag:amb0639)

Predicted SEED Role

"DNA gyrase subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W9N2 at UniProt or InterPro

Protein Sequence (816 amino acids)

>AMB_RS03290 DNA topoisomerase (ATP-hydrolyzing) subunit B (Magnetospirillum magneticum AMB-1)
MSTEPMIPNDPHASANGAEEYGAGSIQVLRGLEAVRKRPGMYIGDTDDGSGLHRMIYEAV
DNAVDEIMNGFGDRCDVVLNADGSVLVTDNGRGIPVDIHPEEGISAAEVALTKLHAGGKF
EQNSNRISSGLHGVGISVVNALSEWLELRIWRHGKEHFMRFRHGEPEAPLAVVGECGDRT
GTQVVFLPSKGTFSHTDFDFDTVEHRLRELAFLNSRATLLIKDERHAETREALLHYEGGV
QAFVQWLDRSKEALHAPILIAAEKDVVRLELSMEWTDSYHETCLCFTNNVPQKDGGTHLA
GFRAALTRTINAYANDSGIAKKEKVNLAGDDMREGLTCVLSVKMPDPKFSSQAKEKLVSS
EVRPLVENLVGEKLAEWFEEHPAEARKVVTKVVEAAAAREAARKARELTRRKGVLDIATL
PGKLADCQERDPALSELFIVEGDSAGGSAKQGRNRANQAILPLKGKILNVERARFDKMLS
SAEIGTLIAAMGTGIGREDFNVDKARYHKIIIMTDADVDGSHIRTLLLTFFYRQMPALIE
RGYLYIAQPPLYRAKRGQSEVYLKDDRALEEYLIDGGLADAVLRMADGTQLASIDLRALV
EQARSVRNLLTPLSRRVPLKLVEQAAIADALDSSLLTDAVRGPAIVQGLAQRLDALETHL
ERGWTGTWVEGDGYTFSRTLRGVTETHALDSAVIRSAEARRLHELAPRLRETFDRPAALM
VRERETVLSGPVALVTAVMEQGRRGIAIQRYKGLGEMNPEQLWETTLDPQARSLLQVKVA
QADDAEQVFSTLMGDVVEPRRDFIQTNALKVSNLDV