Protein Info for AMB_RS03090 in Magnetospirillum magneticum AMB-1

Annotation: tRNA guanosine(34) transglycosylase Tgt

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 375 TIGR00430: tRNA-guanine transglycosylase" amino acids 6 to 364 (359 residues), 492 bits, see alignment E=1.1e-151 TIGR00449: tRNA-guanine family transglycosylase" amino acids 6 to 364 (359 residues), 481.9 bits, see alignment E=1.1e-148 PF01702: TGT" amino acids 13 to 364 (352 residues), 524.7 bits, see alignment E=6e-162

Best Hits

Swiss-Prot: 71% identical to TGT_RHOS4: Queuine tRNA-ribosyltransferase (tgt) from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

KEGG orthology group: K00773, queuine tRNA-ribosyltransferase [EC: 2.4.2.29] (inferred from 100% identity to mag:amb0599)

MetaCyc: 57% identical to tRNA-guanine transglycosylase (Escherichia coli K-12 substr. MG1655)
tRNA-guanine transglycosylase. [EC: 2.4.2.29]

Predicted SEED Role

"tRNA-guanine transglycosylase (EC 2.4.2.29)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 2.4.2.29)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W9S2 at UniProt or InterPro

Protein Sequence (375 amino acids)

>AMB_RS03090 tRNA guanosine(34) transglycosylase Tgt (Magnetospirillum magneticum AMB-1)
MTEFSFELLATDGAARRGRVHTAHGAIDTPAFMPVGTAGTVKAMLPASVAATGAQIVLGN
TYHLMLRPGAERVARLGGLHKFMNWPGPILTDSGGFQVMSLAKLRKMDADGVTFQSHHDG
SKHRLTPESSMEIQRLLDADITMAFDECTPFPATFEQAQESMLLSMRWARRSKEAFVARE
GYGLFGIVQGGVYPDLRAESAKALLEIGFEGYAVGGLAVGEGQEAMFATLEVTTPLLPTD
KPRYLMGVGRPSDIIGAVGRGVDMFDCVMPTRSGRTAQAFTRRGTVNLRNARHQDDPRPL
EEGCPCPACRDHSRAYLHHLIRSEEILGPMLLTWHNIQAYQTLTAGLRAAIAEGRFAQFA
ADAAALEEQGDIAPL