Protein Info for AMB_RS03075 in Magnetospirillum magneticum AMB-1

Annotation: ADP-glyceromanno-heptose 6-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 328 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details TIGR02197: ADP-glyceromanno-heptose 6-epimerase" amino acids 2 to 322 (321 residues), 406.5 bits, see alignment E=3.9e-126 PF04321: RmlD_sub_bind" amino acids 2 to 155 (154 residues), 39.9 bits, see alignment E=8.2e-14 PF01370: Epimerase" amino acids 2 to 249 (248 residues), 133.3 bits, see alignment E=2.9e-42 PF16363: GDP_Man_Dehyd" amino acids 3 to 274 (272 residues), 58.8 bits, see alignment E=2e-19 PF01073: 3Beta_HSD" amino acids 3 to 149 (147 residues), 34.1 bits, see alignment E=4.6e-12

Best Hits

Swiss-Prot: 42% identical to HLDD_RHILO: ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K03274, ADP-L-glycero-D-manno-heptose 6-epimerase [EC: 5.1.3.20] (inferred from 100% identity to mag:amb0596)

Predicted SEED Role

"ADP-L-glycero-D-manno-heptose-6-epimerase (EC 5.1.3.20)" in subsystem LOS core oligosaccharide biosynthesis (EC 5.1.3.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W9S5 at UniProt or InterPro

Protein Sequence (328 amino acids)

>AMB_RS03075 ADP-glyceromanno-heptose 6-epimerase (Magnetospirillum magneticum AMB-1)
MILVTGGAGFIGSNILAALEEKGAGKLVVCDRLRSGTKWQNIAKRELADIVHPEQIFDFL
EANAKHMEVVIHMGAISATTETDADKILANNFSLSLALWKWCALHDVRFIYASSAATYGD
GTQGFDDDGSMEHLAHLRPLNAYGWSKHLFDRRVARKVWAGSRKPPQWAGLKFFNVYGPN
EYHKGGQQSVVAQVYPHAEKNAAYQLFRSHNPKYPDGGQLRDFIWIGDVVDVVMWLVENP
KVSGLFNVGTGKARSFLDLANAVYRAVGREPQIKFRDTPIEIRDKYQYFTQAKMERLHRA
GYSKPFTSLEDGVEQYVKRYLASGDAYR