Protein Info for AMB_RS02740 in Magnetospirillum magneticum AMB-1

Annotation: nitrite reductase (NAD(P)H) small subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 111 PF13806: Rieske_2" amino acids 3 to 100 (98 residues), 52.3 bits, see alignment E=5e-18 PF00355: Rieske" amino acids 3 to 80 (78 residues), 66.8 bits, see alignment E=1.3e-22 TIGR02378: nitrite reductase [NAD(P)H], small subunit" amino acids 4 to 102 (99 residues), 90.3 bits, see alignment E=4.1e-30

Best Hits

Swiss-Prot: 44% identical to NASE_BACSU: Assimilatory nitrite reductase [NAD(P)H] small subunit (nasE) from Bacillus subtilis (strain 168)

KEGG orthology group: K00363, nitrite reductase (NAD(P)H) small subunit [EC: 1.7.1.4] (inferred from 100% identity to mag:amb0532)

Predicted SEED Role

"Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W9Y9 at UniProt or InterPro

Protein Sequence (111 amino acids)

>AMB_RS02740 nitrite reductase (NAD(P)H) small subunit (Magnetospirillum magneticum AMB-1)
MQDWIKIGRVEDIPRLGARTVQTDKGDIAVFRTRDDEIFALFNRCPHKGGPLSEGIVTGK
VVVCPLHSWTIDLETGEATAPDEGCTRPVDSKVVDGLVWLKLESAKKVAHG