Protein Info for AMB_RS02685 in Magnetospirillum magneticum AMB-1
Annotation: glutamate synthase large subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 73% identical to GLTB_AZOBR: Glutamate synthase [NADPH] large chain (gltB) from Azospirillum brasilense
KEGG orthology group: K00265, glutamate synthase (NADPH/NADH) large chain [EC: 1.4.1.13 1.4.1.14] (inferred from 70% identity to acr:Acry_1666)MetaCyc: 73% identical to glutamate synthase alpha subunit (Azospirillum brasilense)
Glutamate synthase (NADPH). [EC: 1.4.1.13]
Predicted SEED Role
"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- ammonia assimilation cycle I (2/2 steps found)
- L-glutamate biosynthesis IV (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- superpathway of ammonia assimilation (plants) (2/3 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
- L-glutamate and L-glutamine biosynthesis (4/7 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14
Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q2WA00 at UniProt or InterPro
Protein Sequence (1504 amino acids)
>AMB_RS02685 glutamate synthase large subunit (Magnetospirillum magneticum AMB-1) MSDFVSQYLANQEILEAAGIDTSPHDACGVGMVAALDGKPRRDVVEAGIQALSVLFHRGA VDADGKTGDGAGIHVEIPQDFFKDHVARTGHAVAEGRLAVGQVFLPKTDLGAQENCRCIV ETEILNFGYSIYGWRQVPVDVSIIGEKANATRPEIEQIMIANTLGTSEERFEADLFVIRR RIEKRVLGNHINDFYICSLSCRSIIYKGMFLAEQLTSFYPDLLDKRFVSRFAIYHQRYST NTFPTWRLAQPFRMLAHNGEINTLTGNINWMKAHEPRMEHEVFGQAMDDLKPVVQPGGSD SAALDNVFEVMCRAGRPAPLVKQMLIPESLGSNALMPEAHRTFFGYCNSVIEPWDGPAAI CATDGQWAVAGVDRNGLRPMRFTITKTGLLILGSETGMVKVKDCDVVEKGRVGPGQSIAI DLDAGKFYRDTEIKDLLASRRNYSAWVKRITELDTLVRTGAPEPAELSSDELRRRQAAYG MTMEDMELILHPMVEDAKEAIGSMGDDTPLAVLSAGYRGLHHFFKQNFSQVTNPPIDSLR ESRVMSLRTRLGNLGNILDEDESQCDLLQLESPVLSNAEFAAMRKYMGEAAAAIDCTFDP KGGIEALRDALARVRRESEEAVRGGCTHLILTDEGIGESRAAIPMILAAGGVHSHLVRLS LRTWTSLNVRSGECLDVHYFAVLIGVGATTVNAYLAQEAIADRQRRGLFGSMPLEECVRR YKYALDQGLLKIMAKMGISVVSSYRGGYNFEAVGLSRTLVAEFFPGMTSRISGIGLSGIQ KKTVEQHATGFAGPAPALPIGGFYRVRRGSEAHSFDGSLIHLLQMAVATGSYATYKKYSD GMRKLPPITIRDLLDLKPAGAPISLDEVESITEIRKRFLTPGMSLGALSPEAHGTLNIAM NRIGAKSVSGEGGEDRERYRPRPNGDNANSAVKQIASGRFGVTAEYLNMCREIEIKVAQG AKPGEGGQLPGFKVTVEIAKLRHATPGVMLISPPPHHDIYSIEDLAQLIYDLKQINPHAR VTVKLVSRSGIGTVAAGVAKAKADTILVSGHVGGTGASPQTSIKFAGLPWELGLSEAHQV LTLNRLRHRVKLRTDGGLKTGRDIVIAAMLGAEEFGIGTSSLVAMGCIMVRQCHSNTCPV GVCTQDLSLREKFTGNPEKVVNLFSFIAEDVREILASLGVRSLAEVIGRADLLHQVSRGA SHLDDLDLNPLLAQADTGGFPRYCVEDKTQEVPDTLDAQMIIDAKPALEDGEKMQLDYNV RNVQRAIGTKLSHKIFKRHGMTGLAPDHVTVRLRGSAGQSLGAFAVQGLTLEVFGDSNDY VGKGLSGGTIIVRPPVASQLKSNENTIIGNTVLYGATAGKLFAAGQAGERFAVRNSGAET VIEGCGSNGCEYMTGGTAVILGPVGANFAAGMTGGMAFVYDSDESFAKRVNPESVIWQRL ETPHWEGVLKALVQEHAAVTGSAWAESLLADWDREVPRFWQVVPKEMLSRLAHPVGRTRV NAAE