Protein Info for AMB_RS02225 in Magnetospirillum magneticum AMB-1

Annotation: methionine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02082: methionine synthase
  • amino acids 9 to 1157 (1149 residues), 1227.3 bits, E=0
• PF02574: S-methyl_trans
  • amino acids 13 to 302 (290 residues), 236.8 bits, E=1.2e-73
• PF00809: Pterin_bind
  • amino acids 344 to 577 (234 residues), 141.9 bits, E=7e-45
• PF02607: B12-binding_2
  • amino acids 631 to 704 (74 residues), 78.1 bits, 1.2e-25
• PF02310: B12-binding
  • amino acids 718 to 825 (108 residues), 68.7 bits, E=1e-22
• PF02965: Met_synt_B12
  • amino acids 911 to 1157 (247 residues), 163.6 bits, E=1.7e-51

Best Hits

KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 100% identity to mag:amb0429)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• folate transformations III (E. coli) (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (15/18 steps found)
• folate transformations II (plants) (9/11 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• folate transformations I (9/13 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (6/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (6/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (5/8 steps found)
• L-methionine salvage from L-homocysteine (1/3 steps found)
• L-methionine biosynthesis I (2/5 steps found)
• superpathway of L-methionine salvage and degradation (7/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.13

Sequence Analysis Tools

See Q2WA92 at UniProt or InterPro

Protein Sequence (1157 amino acids)

>AMB_RS02225 methionine synthase (Magnetospirillum magneticum AMB-1)
MTNILDHLSRHVLLCDGGTGALVQAMNLSVEKDFQGLENCTEILVKSRPDVIRGIHARYF
EAGADMVEADTFGASPITLAEFGIAERAHELNQAAIELAWEAAEQFKGDGRTRFVLGAIG
PGTKLPSLGHIGYDELEAAYVIQAAGQIAGGVSAFLVETCQDPLQIKAAVNGCKIANAAA
GTDVPVFVQVTVETTGTLLVGADIAAAATVVQSLGVPLMGLNCAAGPQEMGEHFKWLIDN
WTGFVSIQPNAGLPELVDGQTRYPLLPAELAVWHERFVSAGANLLGGCCGTTPPHISATN
EMLKRIGNGYRPNPVKRSVHWVPSVASLYTQVPLRQENAFLSIGERCNANGSKKFRDLQD
AEDWDGITAIAREQVKEGSHTLDVCTAFVGRNEVADMTEVVSRLRGAVTTPLVIDSTELP
VLEAGLKLYGGKAILNSINFENGEKDAADRLVLARKFGAAVVALTIDEDGMAKDAAAKLR
IAKRLYDFAVTKHGLPASDLLFDPLTFTICTGNEDDRKLAVETLDAIEMITREMPECGIV
LGLSNVSFGLKPAARQVLNSVFIDHAIKRGMTGAIVHVSKIVPLHTLPEEEVKAAEDLIY
DRDPQALSRYIALFGDRKAAEIKKERPAKAEDRLKQRIIDGDRTGLEDDLAQVMEEGWKP
LDIINTLLLDGMKVVGELFGSGKMQLPFVLQSAETMKASVAFLEPHMEKADGQQKATMVL
ATVKGDVHDIGKNLVDIILTNNGYKVINIGIKQPVAEIIKAAKEHKADAVGMSGLLVKST
VIMRENLEEMTNAGLDVPVLLGGAALTRKYVEEDCSKAYGSGRVAYARDAFDGLDLMAKV
ADGSFDAHVAAKAASPHRPGSPSRTLGEAAQPATRPVDWDEINLRRAELHKDVPAPVPPF
WGARVIESAPLQNLIPFLNETMLYQFHWGYRKQGKSIEEFKAWAHKEIRPVAMDMLKRCA
KEEILRPQAVYGYWKAASDNDSVVLFAEDGTTEVARFPFPRQAKDGGLCIADFFRPVSDP
VRDVIGLQVVTMGKRATEVAQDWFKADKYQDYLYLHGLSVEMAEAMAEYVHKRIRSELGF
AAEDAADMDRLLKQNYRGSRFSFGYPACPRIEDQTQLLSLLGAERIGVTLSEEFQLEPEQ
STSAIVTVHPQAKYFSV