Protein Info for AMB_RS01720 in Magnetospirillum magneticum AMB-1

Annotation: threonine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02824: TGS
  • amino acids 3 to 61 (59 residues), 31.3 bits, 3.3e-11
• TIGR00418: threonine--tRNA ligase
  • amino acids 73 to 632 (560 residues), 695.6 bits, E=3.2e-213
• PF07973: tRNA_SAD
  • amino acids 172 to 220 (49 residues), 54.3 bits, 2.1e-18
• PF00587: tRNA-synt_2b
  • amino acids 319 to 531 (213 residues), 168.1 bits, E=4.2e-53
• PF03129: HGTP_anticodon
  • amino acids 543 to 631 (89 residues), 75.1 bits, E=7.9e-25

Best Hits

Swiss-Prot: 100% identical to SYT_MAGSA: Threonine--tRNA ligase (thrS) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K01868, threonyl-tRNA synthetase [EC: 6.1.1.3] (inferred from 100% identity to mag:amb0340)

Predicted SEED Role

"Threonyl-tRNA synthetase (EC 6.1.1.3)" (EC 6.1.1.3)

MetaCyc Pathways

• tRNA charging (18/21 steps found)
• shinorine biosynthesis (3/8 steps found)
• vanchrobactin biosynthesis (2/8 steps found)
• enterobactin biosynthesis (4/11 steps found)
• superpathway of chorismate metabolism (38/59 steps found)
• acinetobactin biosynthesis (2/9 steps found)
• pseudomonine biosynthesis (2/9 steps found)
• bacillibactin biosynthesis (3/12 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.3

Sequence Analysis Tools

See Q2WAI1 at UniProt or InterPro

Protein Sequence (638 amino acids)

>AMB_RS01720 threonine--tRNA ligase (Magnetospirillum magneticum AMB-1)
MVAITLPDGKVRQFDHPVTGLDVAKDIGPGLAKAALAITIDGEMKDLATLIDRDVNLSII
TAKSGQDALELLRHDAAHVMAEAVKELYPETQVTIGPSIENGFYYDFARPTPFTPDDLAK
IEARMAEIVDRDEAITREEWDRDAAVKFFEDAGEKYKAEIIASIPADQKIGLYRQGNFID
LCRGPHLPSTAKLGKAFKLMKLAGAYWRGDSRNEMLQRIYGTAWFDKKELDAYLHMLEEA
EKRDHRRLGREMELFHQQEEAAGSVFWHKKGWTLYRAVESYMRRRLEANNYEEVKTPQLV
DFSLWEASGHADKFSESMFTIKTQDERHLAVKPMNCPCHVQIFRQGIKSYRDLPLRMAEF
GSCHRYEPSGALHGIMRVRAFTQDDAHIFCTEDQITSETIAFCQLLKEVYTDFGFTDVRV
KFSDRPAKRAGSDETWDKAESALLEASKAAGLETVLNPGEGAFYGPKLEFVLRDAIGRDW
QCGTLQVDFVLPERLDAAYVAEDGAKKRPVMLHRAILGSFERFLGILIENFAGRFPLWLA
PTQVVVATIVSEADDFAREVEATLKAAGLRVELDLRNEKINYKVREHSVAKVPVMLVVGK
REAESRQVAIRRLGSQNQEIVALDQAVATLAAEATPPA