Protein Info for AMB_RS01545 in Magnetospirillum magneticum AMB-1

Annotation: 2,3-bisphosphoglycerate-independent phosphoglycerate mutase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01676: Metalloenzyme
  • amino acids 11 to 501 (491 residues), 275.7 bits, E=3.8e-86
• TIGR01307: phosphoglycerate mutase (2,3-diphosphoglycerate-independent)
  • amino acids 11 to 512 (502 residues), 677 bits, E=7.3e-208
• PF06415: iPGM_N
  • amino acids 88 to 297 (210 residues), 243.6 bits, E=1.7e-76

Best Hits

Swiss-Prot: 60% identical to GPMI_GLUOX: 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI) from Gluconobacter oxydans (strain 621H)

KEGG orthology group: K01834, phosphoglycerate mutase [EC: 5.4.2.1] (inferred from 100% identity to mag:amb0305)

MetaCyc: 53% identical to 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (Escherichia coli K-12 substr. MG1655)
3PGAREARR-RXN [EC: 5.4.2.12]

Predicted SEED Role

"2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.1)" in subsystem Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes (EC 5.4.2.1)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (20/22 steps found)
• Rubisco shunt (10/10 steps found)
• homolactic fermentation (11/12 steps found)
• Bifidobacterium shunt (13/15 steps found)
• glycolysis III (from glucose) (10/11 steps found)
• glycolysis IV (9/10 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (20/26 steps found)
• glycolysis II (from fructose 6-phosphate) (9/11 steps found)
• gluconeogenesis I (10/13 steps found)
• glycolysis I (from glucose 6-phosphate) (10/13 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• glycolysis VI (from fructose) (7/11 steps found)
• glycolysis V (Pyrococcus) (6/10 steps found)
• photorespiration II (6/10 steps found)
• superpathway of glucose and xylose degradation (11/17 steps found)
• glycerol degradation to butanol (10/16 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (12/19 steps found)
• superpathway of hexitol degradation (bacteria) (11/18 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (10/17 steps found)
• Entner-Doudoroff pathway III (semi-phosphorylative) (4/9 steps found)
• superpathway of N-acetylneuraminate degradation (12/22 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Glycolysis / Gluconeogenesis

Isozymes

Compare fitness of predicted isozymes for: 5.4.2.1

Use Curated BLAST to search for 5.4.2.1 or 5.4.2.12

Sequence Analysis Tools

See Q2WAL6 at UniProt or InterPro

Protein Sequence (520 amino acids)

>AMB_RS01545 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (Magnetospirillum magneticum AMB-1)
MSSASPRRPRPVVLCILDGWGWRDDSADNAIAQAETPNWDRFLASYPHSLLQSSGLQVGL
PDGQMGNSEVGHMNLGAGRVVMQDLPRIDAAVADGSLAANPELAKAIAAVKARGGAMHLM
GLLSPGGVHSHQDHLAALARTIADAGVKVQVHAFLDGRDTPPSSAKGFMERFLAAVKGHD
VAVATVSGRYYAMDRDKRWDRVSLAWNALVEAKGVAGADPLAAIAASYAEAKTDEFMLPA
VIGAYQGMRDGDGLLMGNFRADRAREILDCLVNPAFDGFARARQVAFAARLGLTEYSKDL
NAFLGTLFGPETLDNILGEVLSNAGLKQLRIAETEKYAHVTFFFNGGRETVFPGEERILV
PSPKVATYDLQPEMSAAEVTDKLVAAIESGVFDVVVVNYANGDMVGHTGFLKAAIVAAQT
VDACLGRLEAAIAKAGGTMLVTADHGNAEQMKDPTTGEPHTAHTTGPVPAVLVAPPAGIE
GIGDGRLADVAPTLLALLGLAQPAEMSGQSLLRPTLRAAG