Protein Info for AMB_RS01495 in Magnetospirillum magneticum AMB-1

Annotation: sirohydrochlorin chelatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01903: CbiX
  • amino acids 12 to 115 (104 residues), 86 bits, E=2e-28
  • amino acids 142 to 244 (103 residues), 87.1 bits, E=9.6e-29

Best Hits

KEGG orthology group: K03795, sirohydrochlorin cobaltochelatase [EC: 4.99.1.3] (inferred from 100% identity to mag:amb0294)

Predicted SEED Role

"Sirohydrochlorin cobaltochelatase (EC 4.99.1.3)" in subsystem Coenzyme B12 biosynthesis or Experimental tye (EC 4.99.1.3)

MetaCyc Pathways

• cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (14/15 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
• siroheme biosynthesis (3/4 steps found)
• factor 430 biosynthesis (3/7 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.99.1.3

Use Curated BLAST to search for 4.99.1.3

Sequence Analysis Tools

See Q2WAM7 at UniProt or InterPro

Protein Sequence (326 amino acids)

>AMB_RS01495 sirohydrochlorin chelatase (Magnetospirillum magneticum AMB-1)
MSEKIGVMLCGHGSRDVDAIREFQALAGHLTRRLPQYEVESGFLEFATPIIRTSLDALKA
KGVSRILAVPGMLFAAGHVKNDLPWEINSFAAENPGLPITFGRELAIDTKLLAAARARIE
EAEAASNVAIERKDTLLLVVGRGTNDPDANSNIAKVARMLWEGMGFGWAEIAFSGVAYPL
VDQALERVTRLGYKRVVVFPYFLFTGILVKRIYEWTDQAAAAHPGIEFLKAPYLNDHPLV
IDSFVERVGEILDGSPAMNCSLCKYREQVVGYEASVGAVQAGHHHHVRGIGTDEDHGHHH
DHGHGHDHDHGHHHGHGHAYRPHRHD