Protein Info for AMB_RS01215 in Magnetospirillum magneticum AMB-1

Annotation: pyruvate carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1154 PF00289: Biotin_carb_N" amino acids 11 to 120 (110 residues), 136.5 bits, see alignment E=2e-43 TIGR01235: pyruvate carboxylase" amino acids 13 to 1152 (1140 residues), 1793 bits, see alignment E=0 PF02786: CPSase_L_D2" amino acids 126 to 332 (207 residues), 235.3 bits, see alignment E=2.1e-73 PF07478: Dala_Dala_lig_C" amino acids 136 to 302 (167 residues), 28 bits, see alignment E=6e-10 PF02785: Biotin_carb_C" amino acids 353 to 461 (109 residues), 109.7 bits, see alignment E=3.2e-35 PF00682: HMGL-like" amino acids 541 to 812 (272 residues), 102.1 bits, see alignment E=1.5e-32 PF02436: PYC_OADA" amino acids 836 to 1035 (200 residues), 259 bits, see alignment E=1.2e-80 PF00364: Biotin_lipoyl" amino acids 1086 to 1152 (67 residues), 52 bits, see alignment 2.1e-17 PF13533: Biotin_lipoyl_2" amino acids 1089 to 1124 (36 residues), 26.1 bits, see alignment (E = 2.4e-09)

Best Hits

KEGG orthology group: K01958, pyruvate carboxylase [EC: 6.4.1.1] (inferred from 67% identity to amv:ACMV_29950)

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2WAT2 at UniProt or InterPro

Protein Sequence (1154 amino acids)

>AMB_RS01215 pyruvate carboxylase (Magnetospirillum magneticum AMB-1)
MIPSASGKPPFAKLLVANRGEIAIRICRAATELGLATVAVYSTEDRFALHRFKADESYLI
GKGKGPIEAYLSIDEMIRVGKEAGCDAVHPGYGFLSENPDFADAVRAAGMAFVGPSSDVM
RLLGNKVAARALAEKAGVPVMPATGPLPADLVECKRQAAAVGYPLMLKASWGGGGRGMRV
IEAETELEELLAVARREAKAAFGNDEVYLEKLVRRARHVEVQILGDSQGNLVHLYERDCS
VQRRNQKVVERAPAPYLDGRQRSELCETAVRLARAAAYENAGTVEFLMDMETGAFYFIEV
NPRVQVEHTVTEVVTGIDIVKAQIRIAGGAPIGAPGSDVPKQAEIPLNGHAIQCRITTED
PANNFIPDYGRISAYRGANGFGIRLDGGTAFSGALITRYYDSLLEKVTAWAPTPEEAIAR
MDRALREFRIRGVATNLTFLEAVIEHPKFVGCAYTTRFIDETPELFARQDRRDRATKVLS
FVGDVMVNGNPDVAGLKRPAATHRPDLPAFPASEPPAGTRQLLDRLGAEGLAKWMLEQKR
VLITDTTMRDAHQSLFATRMRSHDMLAAAPAYARLLPGLFSVECWGGATFDVAMRFLRED
PWERLRAFRSAMPNLLLQMLLRSANAVGYTNYPDNVVRQFVAQAAENGVDVFRVFDSLNW
VGNMHVAMDAVRESGKLCEAAICYTGDIFDSARPKYDLKYYVGLAKELEKGGAHILGIKD
MAGLARPRAISALVSALKQETGLPIHFHTHDTSGLSAASVLAAVEAGVDAVDAAMDAMSG
LTSQPPLGSICAALAGHERDPGLDQGALNGLSRYWEGVRSLYAPFEADIRSGTAEVYLHE
MPGGQYTNLRAQARSLGVEQHWDWVAKAYADVNAMFGDVVKVTPTSKVVGDMALMMVTSN
LTPADVLDPDKEIAFPESVVSFFKGDLGQPTGGFPAALQKKVLGVAKPITVRPGAVLPPA
DLDAVRAEAEKKAGRKLSDAELASYLMYPKVFADFAAHQRQYGDVSALPTDVFFWGMQPG
QEIAIDLEKGKSLIVRYLATAEAEEDGSRKVFFELNGQPRTVRVFDRKVAPARAARPKAE
AGNADHVGAPMPGLVVSVAVHAGQAVDKGDLLVSIEAMKMETAVRADRAGTVASVAVTPG
VQVEAKDLLVIMAG