Protein Info for AMB_RS01125 in Magnetospirillum magneticum AMB-1

Annotation: phosphomethylpyrimidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 613 transmembrane" amino acids 455 to 473 (19 residues), see Phobius details PF13667: ThiC-associated" amino acids 12 to 83 (72 residues), 105.1 bits, see alignment E=1.4e-34 TIGR00190: phosphomethylpyrimidine synthase" amino acids 127 to 575 (449 residues), 706.6 bits, see alignment E=4.8e-217 PF01964: ThiC_Rad_SAM" amino acids 127 to 571 (445 residues), 620.4 bits, see alignment E=1.5e-190

Best Hits

Swiss-Prot: 100% identical to THIC_MAGSA: Phosphomethylpyrimidine synthase (thiC) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 100% identity to mag:amb0221)

MetaCyc: 73% identical to HMP-P synthase monomer (Caulobacter vibrioides)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

No annotation

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2WAV0 at UniProt or InterPro

Protein Sequence (613 amino acids)

>AMB_RS01125 phosphomethylpyrimidine synthase (Magnetospirillum magneticum AMB-1)
MSETTLKITTGPLPGSRKIYVEGSRPDVRVAMREIDLTPGSGEAPVRVYDCSGPYTDPSM
QVDITKGVPRLREQWILERGDVEHYEGRAHKPEDDGLKPGETIGVPVFDRAGAGLRPLRA
KPGKAPTQLAYARAGIITPEMEYVAIRENMKRAEMKAAVVRDGEDFGADIPDEVTPEFVR
AEIARGRAVLPANVNHPEAEPMIIGRNFLTKINANIGNSAVASSVDEEVEKMVWATRWGA
DTVMDLSTGRHIHATREWIIRNSPVPIGTVPIYQALEKVDGKAEDLTWEIFRDTLIEQAE
QGVDYFTIHAGVLLRYIPLTAKRTTGIVSRGGSIMAKWCLAHHKENFLYTHFEEICELLK
AYDVGFSLGDGLRPGSIADANDAAQFGELETLGELTHKAWAHDCQVIIEGPGHVPMHKIK
KNVEKQIELCGEAPFYTLGPLVTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGL
PDKQDVREGVVTYKLAAHAADLAKGHPGAQVRDNALSRARFEFRWKDQFNLSLDPEKALA
FHDQHLPAEGAKLAHFCSMCGPKFCSMKISQEVRDFAAAEQGMAEMSEKFVNDGAEIYHT
EPAQAQQAKPAAE