Protein Info for AMB_RS01070 in Magnetospirillum magneticum AMB-1

Annotation: hydrogenase 4 subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 669 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 29 to 46 (18 residues), see Phobius details amino acids 56 to 73 (18 residues), see Phobius details amino acids 79 to 99 (21 residues), see Phobius details amino acids 109 to 126 (18 residues), see Phobius details amino acids 130 to 149 (20 residues), see Phobius details amino acids 160 to 182 (23 residues), see Phobius details amino acids 201 to 226 (26 residues), see Phobius details amino acids 238 to 259 (22 residues), see Phobius details amino acids 267 to 290 (24 residues), see Phobius details amino acids 297 to 319 (23 residues), see Phobius details amino acids 337 to 359 (23 residues), see Phobius details amino acids 380 to 404 (25 residues), see Phobius details amino acids 424 to 449 (26 residues), see Phobius details amino acids 470 to 489 (20 residues), see Phobius details amino acids 525 to 546 (22 residues), see Phobius details amino acids 647 to 666 (20 residues), see Phobius details PF00361: Proton_antipo_M" amino acids 126 to 411 (286 residues), 172.3 bits, see alignment E=7.2e-55

Best Hits

KEGG orthology group: K05903, NADH dehydrogenase (quinone) [EC: 1.6.99.5] (inferred from 100% identity to mag:amb0211)

Predicted SEED Role

"Hydrogenase-4 component B / Formate hydrogenlyase subunit 3" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.99.5

Use Curated BLAST to search for 1.6.99.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2WAW0 at UniProt or InterPro

Protein Sequence (669 amino acids)

>AMB_RS01070 hydrogenase 4 subunit B (Magnetospirillum magneticum AMB-1)
MLIAAALSALLALAVLGVAAGAEMWRHRLVYFCCMIASLALLAGGIKHLGQTPGTGPAIV
LPIGLPWMAAHFRLDNLSALFMVVVNLATAAASAYGIGYCSHLPEPRRVTPFYPLFLFGM
NAVLIADDAFMFLVAWEFMSLSSWLLVLADHKNAENRQAAFVYLVMACFGTFCLLTCFGL
MAGGQGAYSFPAMRAAKLDGVAGFLVVLLALLGAGSKAGLVPLHAWLPLAHPAAPSHVSA
LMSGVMTKVALYGLIRILFDLHGEVSWAWGAVMMAIGGITAVMGVLYAILQDDLKKLLAY
STVENIGVVVIGLGLAIAFKGSGEKTLAALALVAGLYHIVNHSIFKTLWFLSAGAVITAT
GERDLGKLGGLTKRMPWNGVAALVGAIAISALPPLNGFVSEWLIFQSLFKGPSLPHWAMK
FGVPVVGAMLALAAALAAACFVRAYGIAFLGRPRSEAAAQAHQVPASMRWTVALLAALCV
VLGAIPVTVTDALSHVVTPLTGVQFAVSADLGWPWLSPVSSTRGSYSGTVLVMTGAGLCA
ITLLLVHGFGTTRIRRADAWDCGHKESIPQSQYTGQSFSQPLRRVFCASIFAARESVDMP
DPGDNRPAELKVRVVDPIWVGLYAGIGRVVDFIADRVNRFQHLTVRHYLLMMFSTLVIML
LVVAVRQSQ