Protein Info for AMB_RS00600 in Magnetospirillum magneticum AMB-1

Annotation: asparagine synthase (glutamine-hydrolyzing)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 560 (559 residues), 422.2 bits, E=1.9e-130
• PF13522: GATase_6
  • amino acids 32 to 160 (129 residues), 123 bits, E=2.1e-39
• PF13537: GATase_7
  • amino acids 48 to 165 (118 residues), 140.5 bits, E=7.3e-45
• PF02540: NAD_synthase
  • amino acids 239 to 321 (83 residues), 22.8 bits, E=1.1e-08
• PF00733: Asn_synthase
  • amino acids 241 to 624 (384 residues), 288.5 bits, E=2.8e-89
• PF06508: QueC
  • amino acids 262 to 319 (58 residues), 24.8 bits, 3.5e-09

Best Hits

KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 100% identity to mag:amb0119)

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• superpathway of L-asparagine biosynthesis (1/2 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.4

Use Curated BLAST to search for 6.3.5.4

Sequence Analysis Tools

See Q2WB52 at UniProt or InterPro

Protein Sequence (639 amino acids)

>AMB_RS00600 asparagine synthase (glutamine-hydrolyzing) (Magnetospirillum magneticum AMB-1)
MCGIFGMVRGGGRPVESNVLAAMKTALHHRGPDGNGIFAEGPVGLGNTRLAVVDTAGGSQ
PVIDPSSGAAIVYNGELFNHLELRAELESVGWCFRTHSDTEVVLAAFCVWGEDCVLRFNG
MYAFAVFDPKARRVFIARDPAGIKPLYLTETADGLAFASEAKALLPIAGRRPDWQALWGY
LTYGYMAPDCSPFAGITKFPAGSLAWIDLELPAARLAVRPYWQPRFGCGAPLAEGEAVDR
LDALLSQAVKHELMSDVPVGLFLSGGLDSSAVAYYAARRHGQAISSFGLAFEETTHDESN
DARTVARHLGIEHKELMFSPALVREGLRRVTETMDEPFGDSTVVPLLMLSRFAREHVTVV
LTGWGGDEVLAGYPTLTAHRVASLYRRLPGMLAHGLVPALVDRLPVSDKYLSFEFKARRF
LRGMKLTPEQQHFLWMGYFDDAFKHALLTPDVTAQVAQQDSLAHFAGMAAGMAEPTLTDR
IMHLDFRYFLHGNGLFQADRMTMAASLEARVPLLNKQMLDFALPLPAGLKMAGGTPKGLL
KKALAPHLPPAILSKPKKGFGPPSGHWVRSVFGPLLDHLFSRERIEAQGILNHATVARLL
AEHRELKADHGRTLWALMSLQLWYDRFILDQASPLERAA