Protein Info for AMB_RS00450 in Magnetospirillum magneticum AMB-1

Annotation: radical SAM protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 378 PF04055: Radical_SAM" amino acids 65 to 241 (177 residues), 46.8 bits, see alignment E=3.8e-16 PF13186: SPASM" amino acids 300 to 367 (68 residues), 63 bits, see alignment E=2.6e-21 TIGR04085: radical SAM additional 4Fe4S-binding SPASM domain" amino acids 300 to 374 (75 residues), 29.3 bits, see alignment E=4.5e-11

Best Hits

KEGG orthology group: None (inferred from 100% identity to mag:amb0089)

Predicted SEED Role

"Predicted Fe-S oxidoreductase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2WB82 at UniProt or InterPro

Protein Sequence (378 amino acids)

>AMB_RS00450 radical SAM protein (Magnetospirillum magneticum AMB-1)
MRHPLPAAAHRLKQDVFPMYDIRFFRASKEIKDDIVAGRYSAKDAPALAARLDAVRDPKP
FVFNMETTNYCNMTCVMCPRTTLMDRKNIWIDDAVFETVLDQVRTHSQTEIEDFWKYIET
TYHVSYQQPDENAFYFYVVSRCLILHGYGEPLLDKKIVDRVRSCSSRNIPTYFSCVPANL
TVERAREVMEAGLSVLKFSMDGLSDEMQKRVRGKRNNFDESFQTILDIIELKREKGYQTL
LVPTMIALSEDDEAKAMHQEFLKLWEGRPVFAYVKSQDNRWYHEEDQDLSNRSHYQSQYC
EYPWTSLTVMADGSVVPCTQDYNVEMVLGNVREQSLEEIWNGKPYADLRRWHVEGTFPDG
HKCSGRCDQKKVCGYLKA