Protein Info for AMB_RS00435 in Magnetospirillum magneticum AMB-1

Annotation: B12-binding domain-containing radical SAM protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 629 transmembrane" amino acids 535 to 555 (21 residues), see Phobius details PF02310: B12-binding" amino acids 12 to 128 (117 residues), 62.4 bits, see alignment E=3.9e-21 PF04055: Radical_SAM" amino acids 226 to 381 (156 residues), 66.8 bits, see alignment E=2.8e-22

Best Hits

KEGG orthology group: None (inferred from 100% identity to mag:amb0086)

Predicted SEED Role

"Fe-S oxidoreductase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2WB85 at UniProt or InterPro

Protein Sequence (629 amino acids)

>AMB_RS00435 B12-binding domain-containing radical SAM protein (Magnetospirillum magneticum AMB-1)
MMNILLITTPIRVRPTNFPPIGALSLLKAARKNGFPSTQFYHVDGNRPDYGAVLEHLEKV
RPDVVGISAVVSTAYAYTKRLCGDIKRLLPDTLVVVGGNLAASAEILLRKAGADLCCIGE
GERVFVNVLRRAETTHRPADFLDIPGLALLDGNGRLRTTSFEAPLGRDEIYDYDIADLVA
ACDDIETYINPLFIDDECQSSEFKFDQMAWQPHRRNKKLAIIPGAKGCVARCTFCHRFDK
GIRYIPVDRIMRQIEILVRDHDVGFISFGDENFGTDKRWLAELCARLKPLDLLWRVGGMR
VNCVSPEIIAMMQDAGCRFIAYGMETGSPRMLEVMEKKTTIEDNRNAIRWTIEAGIAAPI
QIVLGMPGETSETVQETIEFCKHGQTFRPEQNPNDLSINYAQALPGTPLYEFARSRGHIP
LDLDGEERYLLSISDHDAHDEVTTLNLTDYPALICQSWRPRMTVEVNYAYVAKFGLEHYH
KVLLNDANYFKKKRRDDGYFANPKRLVDTSMTVDSMHGSRDAMEVEDENTLPSLWGLLRQ
GNLGLAMICYPILFYRLRRLIPLMILAKNAKRVGLDCAVRQLAETMRWLLRRNWRGGPFT
YKSLRKIVKDDIGPIPGDRDGMDVLRQGR