Protein Info for AMB_RS00405 in Magnetospirillum magneticum AMB-1

Annotation: N-acetylneuraminate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03569: N-acetylneuraminate synthase
  • amino acids 18 to 343 (326 residues), 461.5 bits, E=7.3e-143
• PF03102: NeuB
  • amino acids 38 to 278 (241 residues), 329.7 bits, E=1.1e-102
• PF08666: SAF
  • amino acids 292 to 349 (58 residues), 47.4 bits, E=2.2e-16

Best Hits

Swiss-Prot: 46% identical to NEUBH_CAMJE: N,N'-diacetyllegionaminic acid synthase (legI) from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)

KEGG orthology group: K01654, N-acetylneuraminate synthase [EC: 2.5.1.56] (inferred from 100% identity to mag:amb0080)

MetaCyc: 46% identical to N,N-diacetyllegionaminate synthase (Campylobacter jejuni jejuni NCTC 11168 = ATCC 700819)
RXN-12238 [EC: 2.5.1.101]

Predicted SEED Role

"Legionaminic acid synthase (EC 2.5.1.56)" (EC 2.5.1.56)

MetaCyc Pathways

• CMP-N-acetylneuraminate biosynthesis II (bacteria) (3/3 steps found)
• CMP-legionaminate biosynthesis II (1/3 steps found)
• CMP-legionaminate biosynthesis I (3/10 steps found)
• superpathway of CMP-sialic acids biosynthesis (3/15 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.56

Use Curated BLAST to search for 2.5.1.101 or 2.5.1.56

Sequence Analysis Tools

See Q2WB91 at UniProt or InterPro

Protein Sequence (350 amino acids)

>AMB_RS00405 N-acetylneuraminate synthase (Magnetospirillum magneticum AMB-1)
MIESFTIGSHRVGPGCPCFVIAEAGVNHNGDLALAHRLVDMAVQAGADAVKFQTFTAEKV
VSAQAPKAAYQKVTTGGGQSQLDMVRGFQLPPEAFAELSEHCIQAGILFMSTPFDLDSVD
VLDRLDVPAFKLPSGEITNPLLLRRVAATGRPVILSTGMGSLVEVAAAVDILERAGCHDL
MLLHCTSAYPAPVESANLRAMSTLAAAFHRPVGYSDHTEGFEAAIAAVALGACCIEKHFT
LDRSLPGPDHLASVSPADLGALVAVIRRVEKGLGDGCKRPTVAEEDTRAVARRSLFAARA
LAAGAVMTEEDLIALRPGGGISPMQVDMVVGRRLTRAVAEGTLLEWSDLA