Protein Info for AMB_RS00390 in Magnetospirillum magneticum AMB-1

Annotation: NAD-dependent dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 9 to 171 (163 residues), 43.9 bits, E=7.6e-15
• PF02719: Polysacc_synt_2
  • amino acids 10 to 295 (286 residues), 72.5 bits, E=1.5e-23
• PF01370: Epimerase
  • amino acids 10 to 248 (239 residues), 199.3 bits, E=3.1e-62
• TIGR04180: NAD dependent epimerase/dehydratase, LLPSF_EDH_00030 family
  • amino acids 10 to 306 (297 residues), 521.2 bits, E=3.4e-161
• PF16363: GDP_Man_Dehyd
  • amino acids 11 to 307 (297 residues), 240.1 bits, E=2e-74
• PF01073: 3Beta_HSD
  • amino acids 11 to 230 (220 residues), 87.7 bits, E=3.1e-28
• PF13460: NAD_binding_10
  • amino acids 14 to 140 (127 residues), 49.2 bits, E=2.7e-16
• PF07993: NAD_binding_4
  • amino acids 72 to 189 (118 residues), 36.5 bits, E=1.4e-12

Best Hits

KEGG orthology group: None (inferred from 100% identity to mag:amb0077)

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (11/11 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• stachyose degradation (4/7 steps found)
• D-galactose detoxification (1/3 steps found)
• D-galactose degradation I (Leloir pathway) (2/5 steps found)
• UDP-sugars interconversion (3/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2

Sequence Analysis Tools

See Q2WB94 at UniProt or InterPro

Protein Sequence (339 amino acids)

>AMB_RS00390 NAD-dependent dehydratase (Magnetospirillum magneticum AMB-1)
MSSMPSLKKILVTGADGFIGSHLTEELVRRGYDVRAFALYNSFGSWGWLDAAEPAVRNSL
DVFLGDIRDPHGVRKAMEGCDAVLHLAALIAIPYSYHSPATYVETNVTGTLNVVQAARDL
GVSRVVCTSTSEVYGTARYVPIDEDHPLQGQSPYSATKIGADQMALSYHRSFATPVTVLR
PFNTYGPRQSARAVIPTIITQIAAGARTLKLGALHPTRDFSYVADTAAGFIAMLNAPETV
LGEVINIGSGFEISIGDTARLIAEVMGAQVDITCDDQRLRPEKSEVERLFAGTDKAARLL
GWQPAHGGLEGFRRGLAETVRWFSDPANLARYRADRYTI