Protein Info for ABZR88_RS20230 in Mucilaginibacter yixingensis YX-36 DSM 26809

Annotation: class II fumarate hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00979: fumarate hydratase, class II
  • amino acids 3 to 462 (460 residues), 790.7 bits, E=2.1e-242
• PF00206: Lyase_1
  • amino acids 11 to 343 (333 residues), 359.8 bits, E=1.4e-111
• PF10415: FumaraseC_C
  • amino acids 409 to 461 (53 residues), 94.2 bits, 4.9e-31

Best Hits

Swiss-Prot: 78% identical to FUMC_PASMU: Fumarate hydratase class II (fumC) from Pasteurella multocida (strain Pm70)

KEGG orthology group: K01679, fumarate hydratase, class II [EC: 4.2.1.2] (inferred from 88% identity to shg:Sph21_1523)

MetaCyc: 63% identical to fumarase (Helicobacter pylori 26695)
Fumarate hydratase. [EC: 4.2.1.2]

Predicted SEED Role

"Fumarate hydratase class II (EC 4.2.1.2)" in subsystem TCA Cycle (EC 4.2.1.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• TCA cycle III (animals) (8/10 steps found)
• TCA cycle VIII (Chlamydia) (5/6 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• superpathway of glyoxylate bypass and TCA (9/12 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)
• reductive TCA cycle I (7/11 steps found)
• incomplete reductive TCA cycle (4/7 steps found)
• pyruvate fermentation to propanoate I (4/7 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
• mixed acid fermentation (10/16 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (8/14 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (10/17 steps found)
• methylaspartate cycle (11/19 steps found)
• reductive TCA cycle II (5/12 steps found)
• L-glutamate degradation VIII (to propanoate) (4/11 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (5/13 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (19/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.2

Protein Sequence (465 amino acids)

>ABZR88_RS20230 class II fumarate hydratase (Mucilaginibacter yixingensis YX-36 DSM 26809)
MSFRIEHDTMGEVKVPADKYWGAQTERSRNNFKIGPEASMPKEIIEAFAYLKKAAAYTNT
DLGVLAAEKRDLIAQVCDEILAGELASEFPLVIWQTGSGTQSNMNVNEVVANRAHVLQGN
KLGEGKTFVHPNDDVNKSQSSNDTYPTAMHIAAYKILIDCTIPGVEKLRDTLKAKSEAFK
SVVKIGRTHLMDATPLTLGQEFSGYVSQLDHGLKALRNTFDHLSELALGGTAVGTGINTP
KGYDVKVAEYIAQFTGLPFITAENKFEALAAHDAIVESHGALKQIAVSLMKIANDTRMLA
SGPRSGIGEIHIPDNEPGSSIMPGKVNPTQNEAVTMVAAQVMGNDVAISIGGSNGHYELN
VFKPVMAANFLQSARLIGDACVSFNDHCAKGIEPNYDGIKKHLENSLMLVTALNPHIGYE
NAAKIAKKALKENKSLREAAVELELLSSEQFDQWVRPEDMIGSMK