Protein Info for ABZR88_RS19030 in Mucilaginibacter yixingensis YX-36 DSM 26809

Annotation: thiamine pyrophosphate-dependent enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 658 PF00676: E1_dh" amino acids 18 to 309 (292 residues), 233.4 bits, see alignment E=4.6e-73 PF02779: Transket_pyr" amino acids 345 to 513 (169 residues), 120 bits, see alignment E=1.3e-38 PF02780: Transketolase_C" amino acids 529 to 644 (116 residues), 96.5 bits, see alignment E=1.6e-31

Best Hits

KEGG orthology group: K11381, 2-oxoisovalerate dehydrogenase E1 component [EC: 1.2.4.4] (inferred from 62% identity to cao:Celal_3836)

Predicted SEED Role

"Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4) / Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4)" in subsystem Isoleucine degradation or Leucine Degradation and HMG-CoA Metabolism or Valine degradation (EC 1.2.4.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.4.4

Use Curated BLAST to search for 1.2.4.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (658 amino acids)

>ABZR88_RS19030 thiamine pyrophosphate-dependent enzyme (Mucilaginibacter yixingensis YX-36 DSM 26809)
MIFNRKNLDNNALLSFYKTLLWPRMIEEKMLILLRQGRIGKWFSGIGQEAIAVGATLAMQ
SDEYILPMHRNLGVFTTRNIPLSKLMAQWQGKATGFTKGRDRSFHFGSQQHKIIGMISHL
GPQMALADGIALADVLSNRKKATLVFTGEGATSEGDFHEAINIAAVWKLPVIFLIENNGY
ALSTPTDEQFKCKSLADKATGYGIEGLTIDGNNILDVFYTVRELAASIRENPRPILLECK
TFRMRGHEEASGTKYVPQELFEEWGAKDPLLQFEQYLINEQVLQPEWIELLRKDFSNLIE
REVEAAFNAEDIQVDTDTELADMYAPHAYQPSPLPGNFNEKRFLDAISDAMRLSLRKYPN
LVIMGQDIAQYGGAFKATEGFAEEFGTGRIRNTPLCESGVIGAAMGLSLNGYKAIVEMQF
ADFVSVGFNQIINNLAKTHYRWGQNVDVVLRMPTGAGTSAGPFHSQSNEAWFTKTPGLKV
VYPAFPDDAKGLLMAAINDPNPVIYFEHKYLYRSNSGLVPDGDYTVEIGKARTVRTGTRA
SIITYGWGVHWALEYLEQNPNLSIEIIDLRSLQPWDKQAVEASVKKTGRALILHEDTLTS
GFGGEIAAHLAEHCFQYLDTPVIRCAGLDTAIPMNKQLEDNFLAKARLKESVERLLAY