Protein Info for ABZR88_RS17490 in Mucilaginibacter yixingensis YX-36 DSM 26809

Annotation: dTDP-4-dehydrorhamnose reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 286 PF04321: RmlD_sub_bind" amino acids 3 to 283 (281 residues), 319.6 bits, see alignment E=4.1e-99 TIGR01214: dTDP-4-dehydrorhamnose reductase" amino acids 4 to 281 (278 residues), 302.1 bits, see alignment E=1.7e-94 PF01370: Epimerase" amino acids 5 to 168 (164 residues), 61.3 bits, see alignment E=2.4e-20 PF16363: GDP_Man_Dehyd" amino acids 37 to 153 (117 residues), 32 bits, see alignment E=2.3e-11 PF02719: Polysacc_synt_2" amino acids 37 to 159 (123 residues), 30.8 bits, see alignment E=4.4e-11 PF07993: NAD_binding_4" amino acids 51 to 155 (105 residues), 27 bits, see alignment E=6.2e-10

Best Hits

Swiss-Prot: 39% identical to SPSK_BACSU: Spore coat polysaccharide biosynthesis protein SpsK (spsK) from Bacillus subtilis (strain 168)

KEGG orthology group: K00067, dTDP-4-dehydrorhamnose reductase [EC: 1.1.1.133] (inferred from 66% identity to dfe:Dfer_0045)

Predicted SEED Role

"dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 1.1.1.133)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.133

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (286 amino acids)

>ABZR88_RS17490 dTDP-4-dehydrorhamnose reductase (Mucilaginibacter yixingensis YX-36 DSM 26809)
MSKTIVLGASGQLGQCLKNICEKRGITEFIFPPEEEANILDAEALEKLFVAHQPAYAVNC
AAYTAVDKAEDDVDVARKVNRDGPEIIARLCQKYGIVLVHVSTDFVFKGDEPRLLNEEDV
AEPINIYGLTKLEGEQVITAATDKYYIIRTSWLYSEYGNNFVKAMLKLGAERDELGIIAD
QIGSPTYAIDLAGAIVDIIVSGKDAYGIYHYSNEGVTSWYDFAVGVFDISNTTVKVKPIK
STEYVTKAIRPAFSVMDKSKIKSVFNIAIPYWRESLTTCINILQTS