Protein Info for ABZR88_RS17105 in Mucilaginibacter yixingensis YX-36 DSM 26809

Annotation: NADH-quinone oxidoreductase subunit N

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01770: proton-translocating NADH-quinone oxidoreductase, chain N
  • amino acids 4 to 457 (454 residues), 423.9 bits, E=4.7e-131
• PF00361: Proton_antipo_M
  • amino acids 116 to 409 (294 residues), 242.6 bits, E=2.6e-76

Best Hits

Swiss-Prot: 45% identical to NUON_CYTH3: NADH-quinone oxidoreductase subunit N (nuoN) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K00343, NADH dehydrogenase I subunit N [EC: 1.6.5.3] (inferred from 65% identity to psn:Pedsa_2947)

MetaCyc: 35% identical to ferredoxin-quinone oxidoreductase subunit B (Parasynechococcus marenigrum WH 8102)
RXN-22408 [EC: 7.1.1.10]; Carbonate dehydratase. [EC: 7.1.1.10, 4.2.1.1]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
• CO₂ fixation into oxaloacetate (anaplerotic) (2/2 steps found)
• aerobic respiration I (cytochrome c) (3/4 steps found)
• cyanate degradation (2/3 steps found)
• NADH to cytochrome bd oxidase electron transfer I (1/2 steps found)
• NADH to cytochrome bo oxidase electron transfer I (1/2 steps found)
• C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
• cyclic electron flow (1/3 steps found)
• 3-hydroxypropanoate cycle (8/13 steps found)
• NADPH to cytochrome c oxidase via plastocyanin (thylakoid membrane) (1/4 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• Fe(II) oxidation (2/6 steps found)
• NAD(P)/NADPH interconversion (2/6 steps found)
• C4 photosynthetic carbon assimilation cycle, NAD-ME type (5/11 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• C4 photosynthetic carbon assimilation cycle, PEPCK type (6/14 steps found)
• glyoxylate assimilation (5/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (8/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (19/56 steps found)

KEGG Metabolic Maps

• Nitrogen metabolism
• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3, 4.2.1.1

Use Curated BLAST to search for 1.6.5.3 or 4.2.1.1 or 7.1.1.10

Protein Sequence (465 amino acids)

>ABZR88_RS17105 NADH-quinone oxidoreductase subunit N (Mucilaginibacter yixingensis YX-36 DSM 26809)
MNTLILISVLPILLLYLGLYKAKNALLPVTVIGLAVALGLAISQWKAADTVMPIYNGMML
FDHFSIAFTGITVVSTVLILLLSKDYFEKISSHVAEYYSIILFALAGIIVMVSYHNLTML
FIGIEIMSVSLYILAGIKKQDFGSNEAALKYFLMGAFSTGFLLFGIALIYGTTGSFDLDA
IRDWALSHKSGSYPMFYTGIMLIIVGLCFKVGAAPFHFWTPDVYEGAPTLITAFMSTVVK
TAGFAAFLRLFQNCFVSVAEFWTPVLVIIAGLTLFIGNITALYQQSFKRMLAFSSISHAG
YMLFAIVALGANSGNSVFVYATAYSIASIIAFAVLILVQQQQGNDNFESFNGLGRKNPFL
AFVLTVAMLSLAGIPLTAGFIGKFFMFTGALAKSHIYLVIIAVLNAIISIFYYFRVIIAM
YFRNSEREGLAIPAYFNVVLALSALITLFIGIYPTVIAALIPSVK