Protein Info for ABZR88_RS13440 in Mucilaginibacter yixingensis YX-36 DSM 26809

Annotation: 3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02737: 3HCDH_N
  • amino acids 9 to 198 (190 residues), 148.3 bits, E=4.6e-47
• PF00725: 3HCDH
  • amino acids 201 to 300 (100 residues), 45.3 bits, E=2e-15
• PF00378: ECH_1
  • amino acids 487 to 621 (135 residues), 41.5 bits, E=2.2e-14
• PF16113: ECH_2
  • amino acids 489 to 621 (133 residues), 30.5 bits, E=5.4e-11

Best Hits

KEGG orthology group: K07516, 3-hydroxyacyl-CoA dehydrogenase [EC: 1.1.1.35] (inferred from 66% identity to psn:Pedsa_2594)

Predicted SEED Role

"Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation or Isobutyryl-CoA to Propionyl-CoA Module (EC 1.1.1.35, EC 4.2.1.17)

MetaCyc Pathways

• benzoyl-CoA biosynthesis (3/3 steps found)
• L-isoleucine degradation I (4/6 steps found)
• fatty acid salvage (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• adipate biosynthesis (3/5 steps found)
• adipate degradation (3/5 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• propanoate fermentation to 2-methylbutanoate (3/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
• valproate β-oxidation (5/9 steps found)
• glycerol degradation to butanol (10/16 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (2/5 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (2/5 steps found)
• pyruvate fermentation to butanol I (4/8 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• fatty acid β-oxidation I (generic) (3/7 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• pyruvate fermentation to butanoate (3/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (8/14 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (8/14 steps found)
• oleate β-oxidation (23/35 steps found)
• 6-gingerol analog biosynthesis (engineered) (2/6 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (1/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (16/26 steps found)
• L-valine degradation I (3/8 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (5/11 steps found)
• benzoyl-CoA degradation I (aerobic) (2/7 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (4/10 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
• phenylacetate degradation I (aerobic) (3/9 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (3/9 steps found)
• 2-methylpropene degradation (2/8 steps found)
• 3-phenylpropanoate degradation (3/10 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (5/13 steps found)
• superpathway of phenylethylamine degradation (3/11 steps found)
• methyl tert-butyl ether degradation (2/10 steps found)
• L-glutamate degradation VII (to butanoate) (3/12 steps found)
• L-tryptophan degradation III (eukaryotic) (5/15 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (5/17 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (3/16 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (3/17 steps found)
• toluene degradation VI (anaerobic) (3/18 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
• androstenedione degradation I (aerobic) (6/25 steps found)
• platensimycin biosynthesis (6/26 steps found)
• Spodoptera littoralis pheromone biosynthesis (3/22 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (2/22 steps found)
• superpathway of testosterone and androsterone degradation (6/28 steps found)
• androstenedione degradation II (anaerobic) (4/27 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (8/42 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (8/47 steps found)
• superpathway of cholesterol degradation III (oxidase) (4/49 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Bile acid biosynthesis
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.17

Use Curated BLAST to search for 1.1.1.35 or 4.2.1.17

Protein Sequence (793 amino acids)

>ABZR88_RS13440 3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase family protein (Mucilaginibacter yixingensis YX-36 DSM 26809)
MDAKRIIRKVAVLGSGVMGSRIACHFANIGLQVLLLDIVPEGAAAGDKKARNKIVDDALA
FALKSNPSPIYLKSFASRITTGNFDDDMPKIAECDWVIEVVVERLDIKQKVFEKVDKYRK
AGTLVTSNTSGIPIHLMAQGRSDDFKQHFCGTHFFNPPRYLRLLEIIPTADTSKEVAGFL
TEYGEKYLGKTTVLAKDTPAFIGNRIGIFSIMTVLHYVDKTGMTVEEVDKLTGPAIGRPK
SATFRTNDVVGLDTLVHVANMLSQNAPDAELGELCKMPDFINTMMVNKWLGSKTGQGFYK
KEKTATGSEIYALDLKTLEYKPSQKVKFASVETAKTVDSLKDRLKILVGAKDKAGDFYRA
TFFALFAYSSNRIPEIADELYKIDAAMNAGFGWEMGPFETWDALGVAGTIKEMEAAGSIP
AQWVYDMLASGAESFYKTEDGMRLYYDIQSKSYKAIPGTENYILLDNIRPTKTIWKNSGT
TVTDIGDGILNLEFHTKMNTIGSEVIEGINKAITLAEESYRGLVVSNEGANFSAGANVGL
ILMLAVEQEFDELNMVVKAFQDTMMRVRYSSIPVVVAPHQMTLGGGCEICLHADKVVAHA
ELYMGLVEFGVGLIPAGGGSKEFALRLSDELQEGDIELNNFKERFLTIGQAKVSTSAYEA
FDLGYLKKGRDVVVMSRNRLLAEAKRQCLVLADEGYVQPAPRKDIRVLGKQSLGLAYVGA
NSMFSGNYISEHDVKISQKLAYVLSGGDLSQPSPVSEQYLLNLEREAFVSLCAERKTLER
IQSILTGGKILRN