Protein Info for ABZR88_RS09210 in Mucilaginibacter yixingensis YX-36 DSM 26809

Annotation: DEAD/DEAH box helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 PF04851: ResIII" amino acids 24 to 187 (164 residues), 31.6 bits, see alignment E=2.3e-11 PF00270: DEAD" amino acids 26 to 192 (167 residues), 167.8 bits, see alignment E=2.7e-53 PF00271: Helicase_C" amino acids 229 to 339 (111 residues), 99.7 bits, see alignment E=1.7e-32

Best Hits

Swiss-Prot: 44% identical to RHLE_ECOLI: ATP-dependent RNA helicase RhlE (rhlE) from Escherichia coli (strain K12)

KEGG orthology group: K11927, ATP-dependent RNA helicase RhlE [EC: 3.6.4.13] (inferred from 72% identity to psn:Pedsa_1709)

MetaCyc: 44% identical to ATP-dependent RNA helicase RhlE (Escherichia coli K-12 substr. MG1655)
5.6.2.e [EC: 5.6.2.e]

Predicted SEED Role

"ATP-dependent RNA helicase RhlE" in subsystem ATP-dependent RNA helicases, bacterial

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13 or 5.6.2.e

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (448 amino acids)

>ABZR88_RS09210 DEAD/DEAH box helicase (Mucilaginibacter yixingensis YX-36 DSM 26809)
MASFEDFKFNRQILNAIADSGYTEPTPIQQKAIPPILNGQDVMGIAQTGTGKTAAYVLPL
VMKLKYAQGEHARALILSPTRELAMQIEENIRKYSAYTDLRTVVVYGGIGPKTQIEQINK
GVDIIVATPGRFMDIYLAGHIQTKPLQVLVLDEADKMMDMGFMPQINRILEVVPRKRQNL
LFSATMSDKVHNLSANFLEFPTVVEVTPQATPAQTVKQQLYHVPNIKTKINLLKHLLDEE
GNISKLIVFCKTRTAAEDVYKYLSRRFGEKDVRVLHANKGQNTRINSINSFKEDEVKILV
ATDVASRGIDVSNVSHVINFDVPVVIEDYVHRIGRTGRALQAGEAITFCNPAEEYYVAKI
EKLIRQTIQVSPLPENVFVEQTPFEERQDQAREIDLQKRKENPDFKGAFHEKKTATQRKK
FDAEKAKRNPRVAATKTKGLFKKNKKKH