Protein Info for ABZR88_RS08840 in Mucilaginibacter yixingensis YX-36 DSM 26809

Annotation: phosphomethylpyrimidine synthase ThiC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 16 to 96 (81 residues), 117.5 bits, E=1.8e-38
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 135 to 594 (460 residues), 727.2 bits, E=2.8e-223
• PF01964: ThiC_Rad_SAM
  • amino acids 137 to 590 (454 residues), 629.9 bits, E=1.9e-193

Best Hits

Swiss-Prot: 77% identical to THIC_NITOC: Phosphomethylpyrimidine synthase (thiC) from Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / NCIMB 11848 / C-107)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 87% identity to phe:Phep_2466)

MetaCyc: 70% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (9/10 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (4/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Protein Sequence (627 amino acids)

>ABZR88_RS08840 phosphomethylpyrimidine synthase ThiC (Mucilaginibacter yixingensis YX-36 DSM 26809)
MKIEKVPTDHVITTSPFPASRKVYVTGQLHPIKVAMREISLTDTKIYGRFGETEPNAPVT
VYDTSGPYTDPDMEIDVRKGLQRLRESWIVERGDVEQLDTISSDYGRQRLNNTELDYLRF
GHQSTPYRAKPGQNVSQLHYAKKGIITPEMEYIAIRENQRIDLLKEQLNGQYDVLGQQHE
GHSFGANTPKGYITPEFVRQEVAAGRAVIPSNINHPESEPMIIGRNFLVKINANIGNSAV
TSSIEEEVEKAVWACRWGADTIMDLSTGKNIHETREWIIRNTPVPIGTVPIYQALEKVNG
KAEDLTWELFRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAKRVTGIVSRGGSIMAKWCLA
HHKENFLYTHFEEICEIMKAYDVAFSLGDGLRPGSIADANDAAQFAELETLGELTQIAWK
HDVQTIIEGPGHVPMHMIKENMNKQLKHCAEAPFYTLGPLTTDIAPGYDHITSAIGAAMI
GWFGTAMLCYVTPKEHLGLPNKKDVKDGVITYKIAAHAADLAKGHPGAQYRDNALSKARF
EFRWEDQFNLSLDPDTAREFHDETLPADGAKVAHFCSMCGPNFCSMKITQDVRDYAKDNG
LDSDEALQKGMAEKSKEFAEKGSEIYL