Protein Info for ABZR88_RS03255 in Mucilaginibacter yixingensis YX-36 DSM 26809

Annotation: pseudouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 558 PF00849: PseudoU_synth_2" amino acids 364 to 511 (148 residues), 101 bits, see alignment E=3.9e-33

Best Hits

KEGG orthology group: K06177, ribosomal large subunit pseudouridine synthase A [EC: 5.4.99.12] (inferred from 63% identity to cpi:Cpin_4402)

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70)" in subsystem Ribosome biogenesis bacterial (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (558 amino acids)

>ABZR88_RS03255 pseudouridine synthase (Mucilaginibacter yixingensis YX-36 DSM 26809)
MDGKVIYFNESKISGISLPGRFTFPFFYEPHPLAKIAADDLKHYLENQARLDHNFGLDSD
AQGMAIGKMFGVLIIQDGDGRQGYLSAFSGKLANSNDHPRFVPPVFDMLAEDSFFLKEQY
VLNDINGQVKDIETNQDYLTLQHELKGLTAQTEKQIGDFKKLLKANKADRKKQRDEQKAT
LTPEAYMEFEAYMVKHSLHDKHQLNVLVGDCEARLNKLRQQLAPFEDRLEALKKERKEKS
AALQQQLFEQYAFLNQYGHTRSLYDIFSETVFGTPPSAAGECATPKLLQYAFLNGYKPLA
MAEFWWGASPKSEIRAHGQFYPACTGKCKPILKHMLEGIELDEDPLLVNPGENKPLDIIF
EDDYFVIVNKPSGLLSVPGINISDSVYSRLKQTFKTIEPLIIHRLDMATSGLLVVAKTRE
AHKLIQQQFLKRTVTKRYTALLSKIIEGQEGEINLPLRGDLDDRPRQLVCFEHGKKAITR
WKVIEYKDGKTKIHFWPLTGRTHQLRVHSAHEQGLNAAIVGDDLYGTAAERLYLHAAYLE
FVHPASGEKMSFEVKEDF