Protein Info for ABIE41_RS23160 in Bosea sp. OAE506

Annotation: phosphoribosylamine--glycine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02844: GARS_N
  • amino acids 1 to 100 (100 residues), 127.5 bits, E=4.9e-41
• TIGR00877: phosphoribosylamine--glycine ligase
  • amino acids 1 to 420 (420 residues), 516.7 bits, E=2.2e-159
• PF01071: GARS_A
  • amino acids 101 to 292 (192 residues), 249.2 bits, E=4.6e-78
• PF02843: GARS_C
  • amino acids 327 to 418 (92 residues), 107.5 bits, E=5e-35

Best Hits

Swiss-Prot: 68% identical to PUR2_RHILO: Phosphoribosylamine--glycine ligase (purD) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K01945, phosphoribosylamine--glycine ligase [EC: 6.3.4.13] (inferred from 69% identity to rpe:RPE_4612)

MetaCyc: 56% identical to phosphoribosylamine--glycine ligase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylamine--glycine ligase. [EC: 6.3.4.13]

Predicted SEED Role

"Phosphoribosylamine--glycine ligase (EC 6.3.4.13)" in subsystem De Novo Purine Biosynthesis (EC 6.3.4.13)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (41/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (20/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (4/5 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis II (4/5 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (4/6 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.13

Protein Sequence (426 amino acids)

>ABIE41_RS23160 phosphoribosylamine--glycine ligase (Bosea sp. OAE506)
MKILLIGSGGREHALAWAISASPLCDRLFIAPGNPGTAQCGENVVIDIADHGAVASFCKL
QGIGLVVVGPEGPLVAGLADDLVATGLKVFGPSKAAAQLEGSKAFTKELCAEFAIPTAGF
GRFADAASAKAYVASQGAPIVIKADGLAAGKGVVMAETLDEANEAIEMMFGGGLGAAGAA
IVIEEWMIGEEASFFALCDGTNALALASAQDHKRVGDGDTGPNTGGMGAYSPAPVMTAAM
EARVMKEIIAPTLAGMAQRGTPFKGVLFAGLMITAQGPKLIEYNVRFGDPECEVLMPRLK
SDIVPALLAACDGVLDNITLRWSDEAALTVVLAARGYPGKPETGSVIKEIDKAAALDDVL
VFHAGTKQTATDIVASGGRVLNVVGLGKTVSEAQARAYRGVDAIDWPEGFCRRDIGWQAV
KREREG