Protein Info for ABIE41_RS20910 in Bosea sp. OAE506

Annotation: glutamate--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00464: glutamate--tRNA ligase
  • amino acids 12 to 331 (320 residues), 295.9 bits, E=2.9e-92
• PF00749: tRNA-synt_1c
  • amino acids 13 to 314 (302 residues), 231.6 bits, E=1.1e-72
• PF19269: Anticodon_2
  • amino acids 388 to 448 (61 residues), 39.6 bits, E=7.3e-14

Best Hits

Swiss-Prot: 74% identical to SYE2_METS4: Glutamate--tRNA ligase 2 (gltX2) from Methylobacterium sp. (strain 4-46)

KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 74% identity to met:M446_2622)

Predicted SEED Role

"Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24)" in subsystem tRNA aminoacylation, Glu and Gln (EC 6.1.1.24)

MetaCyc Pathways

• tRNA charging (19/21 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (27/33 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• superpathway of heme b biosynthesis from glutamate (6/10 steps found)
• tetrapyrrole biosynthesis I (from glutamate) (3/6 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (23/36 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Glutamate metabolism
• Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.17

Use Curated BLAST to search for 6.1.1.17 or 6.1.1.24

Protein Sequence (452 amino acids)

>ABIE41_RS20910 glutamate--tRNA ligase (Bosea sp. OAE506)
MTSPAPSRPPLLRFAPSPTGYLHIGNARSALFNGLYARRHAGRFMLRFDDTDLARSKPEY
AAAIAEDLAWLGIVPDLVISQSQRLSLYDAAAEDLRAAGRLYPCYETADELERRRKRQLA
RGLPPIYDRAALKLTAEDRAKLEAEGRRPHWRFLLEPRVVAWTDLIRGDAHVDCASLSDQ
VLIREDGSYLYHLPSVVDDAATGVTHIIRGEDHVTNTAVHIQIFQALGAALPAFGHHNLL
TTASGEGLSKRLGHLSLRGLREAGVEPLAVAALATLTGSSDAVRPVASLDELAGLVDLAH
VSRAPAKFDEHDLDTLSARTLHQLPYAAVAPRLAELGIGGAEPFWLAIRGNLSKLDEARL
WDDVVRGPIRPVVADESFAETAASLLPPEPFDATTWKSWTAAVQAATGTKGKALFMPLRQ
ALTAQDHGPELAALLPLIGRDKALRRLKGESA