Protein Info for ABIE41_RS19865 in Bosea sp. OAE506

Annotation: DNA topoisomerase IV subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 748 TIGR01062: DNA topoisomerase IV, A subunit" amino acids 14 to 746 (733 residues), 854.4 bits, see alignment E=3.2e-261 PF00521: DNA_topoisoIV" amino acids 37 to 475 (439 residues), 496.2 bits, see alignment E=8.6e-153 PF03989: DNA_gyraseA_C" amino acids 609 to 637 (29 residues), 16.3 bits, see alignment (E = 5.2e-07) amino acids 657 to 692 (36 residues), 16.9 bits, see alignment (E = 3.4e-07)

Best Hits

Swiss-Prot: 65% identical to PARC_RHIME: DNA topoisomerase 4 subunit A (parC) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K02621, topoisomerase IV subunit A [EC: 5.99.1.-] (inferred from 72% identity to mrd:Mrad2831_2027)

Predicted SEED Role

"Topoisomerase IV subunit A (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (748 amino acids)

>ABIE41_RS19865 DNA topoisomerase IV subunit A (Bosea sp. OAE506)
MGKPVDPPPEEESERIDLKAALEERYLAYALSTIMHRALPDARDGLKPVHRRILHAMRLL
RLDPGQVHKKCARIVGDVIGKFHPHGDQSVYDALVRLAQDFAQRYPLVDGQGNFGNIDGD
NAAAYRYTEARMTEVARLLLDGIDEDAVDFRETYNGEDEEPIVLPAAFPNLLANGSQGIA
VGMATSIPPHNAAELCDAALYLIQHPETSSEQLMTFVQGPDFPTGGIIVESRASMAETYR
TGRGAFRTRARWHAEDLGRGTWHVVVTEIPYMVQKGRLIEKIAELLNDKKLPLVADIRDE
SAEDIRVVIEPRARNVDPALMMESLFRLTELESRISMNMNVLTGGLVPRVLGLNEALRAW
LDHRRDVLLRRSRFRLGQIEKRLEILRGLLIVYLDLDRVIKIIREEDEPKIELMRVFELN
EVQANAILDTRLRALRKLEEMQLKTELDELTKEKGQIEELLASEPVQWKLIQHDIREVKK
LFGQETAIGKRRTSFADMPDTTDIDLTQAMVEREPVTVVISKKGWIRALKGHVQDQSTLT
FKGDDGLKTAFFTETTAKLLVLATNGKVFTLEAAKLPGGRGFGDPIRLMIDLEEHAEIVE
AFPYRPGLKLLMVTTDGRGFVVPADDVVANTRKGRQVLNVDMPVEALLATPAEGDHVAII
GQNRKLNCFPLSEVAEMSRGKGVRLQRYKDGGVSDAKTFKLADGLTWRDTSGRTWTVAQA
ELMEWLGHRGEAGRLPPKGFPKNNKFGG