Protein Info for ABIE41_RS18055 in Bosea sp. OAE506

Annotation: ATP-dependent Clp protease ATP-binding subunit ClpX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 TIGR00382: ATP-dependent Clp protease, ATP-binding subunit ClpX" amino acids 10 to 408 (399 residues), 664.5 bits, see alignment E=3.3e-204 PF06689: zf-C4_ClpX" amino acids 14 to 50 (37 residues), 70.9 bits, see alignment 2.6e-23 PF07724: AAA_2" amino acids 111 to 307 (197 residues), 121 bits, see alignment E=2.2e-38 PF01078: Mg_chelatase" amino acids 111 to 144 (34 residues), 20.5 bits, see alignment 1.1e-07 PF07728: AAA_5" amino acids 113 to 190 (78 residues), 24.1 bits, see alignment E=1.3e-08 PF00004: AAA" amino acids 114 to 231 (118 residues), 59.4 bits, see alignment E=2.2e-19 PF10431: ClpB_D2-small" amino acids 314 to 385 (72 residues), 45.8 bits, see alignment E=2.1e-15

Best Hits

Swiss-Prot: 90% identical to CLPX_METRJ: ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) from Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 / 0-1)

KEGG orthology group: K03544, ATP-dependent Clp protease ATP-binding subunit ClpX (inferred from 90% identity to mrd:Mrad2831_5135)

MetaCyc: 72% identical to ATP-dependent Clp protease ATP-binding subunit ClpX (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpX" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (422 amino acids)

>ABIE41_RS18055 ATP-dependent Clp protease ATP-binding subunit ClpX (Bosea sp. OAE506)
MSKVSGGDSKSTLYCSFCGKSQHEVRKLIAGPTVFICDECVELCMDIIREESKSALVKSK
DGVPTPKEIRKVLDDYVIGQEHAKKVLSVAVHNHYKRLSHATKHNDVELAKSNILLIGPT
GSGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDVENIILKLLQASDYNVERAQRGIV
YIDEIDKISRKSDNPSITRDVSGEGVQQALLKIMEGTVASVPPQGGRKHPQQEFLQVDTT
NILFICGGAFAGLDKIIAGRGKGTSIGFGATVKGPDERRTGAIFREVEPEDLLKFGLIPE
FVGRLPVLATLEDLDEAALKTILTEPKNALVKQYQRLFEMEDTELTFTDEAVSLIARKAI
ERKTGARGLRSIMEGILLETMYELPGLEGVEQVVIGPEAVESKSRPLFIYSEREEEAKSA
SA