Protein Info for ABIE41_RS14130 in Bosea sp. OAE506

Annotation: CoA ester lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03328: HpcH_HpaI
  • amino acids 2 to 229 (228 residues), 129.8 bits, E=9.3e-42
• PF15617: C-C_Bond_Lyase
  • amino acids 202 to 288 (87 residues), 30.9 bits, E=1.6e-11

Best Hits

KEGG orthology group: K01644, citrate lyase subunit beta / citryl-CoA lyase [EC: 4.1.3.34 4.1.3.6] (inferred from 66% identity to met:M446_2314)

Predicted SEED Role

"Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 4.1.3.4)

MetaCyc Pathways

• citrate degradation (2/2 steps found)
• ketogenesis (4/5 steps found)
• L-leucine degradation I (4/6 steps found)
• mevalonate degradation (1/2 steps found)
• reductive TCA cycle II (6/12 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Citrate cycle (TCA cycle)
• Synthesis and degradation of ketone bodies
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.34, 4.1.3.6

Use Curated BLAST to search for 4.1.3.34 or 4.1.3.4 or 4.1.3.6

Protein Sequence (292 amino acids)

>ABIE41_RS14130 CoA ester lyase (Bosea sp. OAE506)
MRSLLFVPGDSPRKQQKGLTSGADALILDLEDSVALDAKPQAREITRAFLAEARALPRCP
RLIVRVNALTTGLTEADLDAVMPGAPDAIMLPKSESGSDAGHLAAKIAVREAECDLPDGG
TRIIPIATETGKGIFGLGSYAGATHRLEALTWGAEDLSADLGAETNRLPDGHYTDPYRLA
RSLTLFAAASAQVDAIDTVFTAFRDAEGFRAECLAARRDGFTGKMAIHPDQVAPINEVFS
PSPEALARAQAIIALFEANPGLGVIGLDGEMLDRPHLVRAQRLVARARRWDE